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Article

Entomological Assessment of Onchocerca Species Transmission by Black Flies in Selected Communities in the West Region of Cameroon

1
Department of Gerontology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Laarbeeklaan 103, B-1090 Brussels, Belgium
2
Frailty in Ageing Research Group, Vrije Universiteit Brussel, Laarbeeklaan 103, B-1090 Brussels, Belgium
3
Department of Biochemistry and Molecular Biology, Faculty of Science, University of Buea, Buea P.O. Box 63, Cameroon
4
Department of Public Health and Hygiene, Faculty of Health Science, University of Buea, Buea P.O. Box 63, Cameroon
5
Department of Molecular Biology, Institute of Biology and Molecular Medicine, IBMM, Universite Libre de Bruxelles, Gosselies Campus, 126040 Gosselies, Belgium
*
Authors to whom correspondence should be addressed.
These authors are last authors on this work.
Pathogens 2020, 9(9), 722; https://doi.org/10.3390/pathogens9090722
Received: 13 August 2020 / Revised: 29 August 2020 / Accepted: 31 August 2020 / Published: 2 September 2020
(This article belongs to the Special Issue Onchocerciasis and River Epilepsy)
The enormity of the public health burden of onchocerciasis motivated the creation of various large-scale control programs that have depended principally on mass treatment of endemic communities with ivermectin for the elimination of the disease. Parasitological evaluation of Onchocerca species in the West Region of Cameroon indicates significant progress in the interruption of parasite transmission in some communities under ivermectin treatment. However, to verify the complete elimination of onchocerciasis, entomological assessment through O-150 PCR poolscreen of black flies is mandatory. Thus, in the present study, we assessed transmission of Onchocerca species using an O-150 PCR technique to screen pools of black flies—in seven onchocerciasis endemic communities (Makouopsap, Bankambe, Lemgo, Tsesse, Ndionzou, Kouffen, and Bayon) in Cameroon. Two thousand black flies were assessed—in each community—for the presence of Onchocerca species DNA. Our results show that the frequency of infective flies was 0.6% in Makouopsap and 0.0% in the other communities. On the other hand, the frequency of infected flies was 0.8% in Makouopsap, 0.2% in Bankambe, 0.1% in Bayon, and 0.0% in Lemgo, Tsesse, Ndionzou, and Kouffen. These results provide entomologic evidence for continuous transmission of Onchocerca species in Makouopsap, risk of active transmission in Bankambe, and Bayon, and a suppressed transmission in the four other studied communities. View Full-Text
Keywords: onchocerciasis; ivermectin; O-150 PCR; poolscreen; elimination onchocerciasis; ivermectin; O-150 PCR; poolscreen; elimination
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MDPI and ACS Style

Shintouo, C.M.; Nguve, J.E.; Asa, F.B.; Shey, R.A.; Kamga, J.; Souopgui, J.; Ghogomu, S.M.; Njemini, R. Entomological Assessment of Onchocerca Species Transmission by Black Flies in Selected Communities in the West Region of Cameroon. Pathogens 2020, 9, 722. https://doi.org/10.3390/pathogens9090722

AMA Style

Shintouo CM, Nguve JE, Asa FB, Shey RA, Kamga J, Souopgui J, Ghogomu SM, Njemini R. Entomological Assessment of Onchocerca Species Transmission by Black Flies in Selected Communities in the West Region of Cameroon. Pathogens. 2020; 9(9):722. https://doi.org/10.3390/pathogens9090722

Chicago/Turabian Style

Shintouo, Cabirou M.; Nguve, Joel E.; Asa, Fru B.; Shey, Robert A.; Kamga, Joseph; Souopgui, Jacob; Ghogomu, Stephen M.; Njemini, Rose. 2020. "Entomological Assessment of Onchocerca Species Transmission by Black Flies in Selected Communities in the West Region of Cameroon" Pathogens 9, no. 9: 722. https://doi.org/10.3390/pathogens9090722

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