Next Article in Journal
S-methyl Methanethiosulfonate: Promising Late Blight Inhibitor or Broad Range Toxin?
Previous Article in Journal
Actinotignum schaalii Abscess in a Patient with Common Variable Immunodeficiency
Previous Article in Special Issue
Focus of Ongoing Onchocerciasis Transmission Close to Bangui, Central African Republic
Open AccessArticle

In Silico Design and Validation of OvMANE1, a Chimeric Antigen for Human Onchocerciasis Diagnosis

1
Department of Gerontology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Laarbeeklaan 103, B-1090 Brussels, Belgium
2
Frailty in Ageing Research Group, Vrije Universiteit Brussel, Laarbeeklaan 103, B-1090 Brussels, Belgium
3
Department of Biochemistry and Molecular Biology, Faculty of Science, University of Buea, P.O. Box 63 Buea, Cameroon
4
Department of Molecular Biology, Institute of Biology and Molecular Medicine, IBMM, Université Libre de Bruxelles, Gosselies Campus, 126040 Gosselies, Belgium
5
Division of Human Genetics, Department of Pathology, University of Cape Town, Health Sciences Campus, Anzio Rd, Observatory 7925, South Africa
6
Department of Microbiology and Parasitology, Faculty of Science, University of Buea, P.O. Box 63 Buea, Cameroon
7
Department of Parasitology and Medical Entomology, Centre for Research in Infectious Diseases (CRID), Yaounde, Cameroon
8
Laboratory of Medical and Molecular Oncology, Oncology Research Center, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Laarbeeklaan 103, B-1090 Brussels, Belgium
9
Centre for Human Genetics, College of Medicine and Health Sciences, University of Rwanda, KG11 Ave, Kigali 4285, Rwanda
*
Authors to whom correspondence should be addressed.
These authors are last authors on this work.
Pathogens 2020, 9(6), 495; https://doi.org/10.3390/pathogens9060495
Received: 22 May 2020 / Revised: 12 June 2020 / Accepted: 18 June 2020 / Published: 22 June 2020
(This article belongs to the Special Issue Onchocerciasis and River Epilepsy)
The public health goal of onchocerciasis in Africa has advanced from control to elimination. In this light, accurate diagnosis is necessary to determine treatment endpoints and confirm elimination, as well as to conduct surveillance for the identification of any possible recrudescence of the disease. Currently, the monitoring of onchocerciasis elimination relies on the Ov-16 test. However, this test is unable to discriminate between past and active infections. Furthermore, about 15–25% of infected persons are reported to be negative for the Ov-16 test, giving a misleading sense of security to false-negative individuals who might continue to serve as reservoirs for infections. Therefore, we opted to design and validate a more sensitive and specific chimeric antigen (OvMANE1) for onchocerciasis diagnosis, using previously reported immunodominant peptides of O. volvulus, the parasite responsible for the disease. In silico analysis of OvMANE1 predicted it to be more antigenic than its individual peptides. We observed that OvMANE1 reacts specifically and differentially with sera from O. volvulus infected and non-infected individuals, as well as with sera from communities of different levels of endemicity. Moreover, we found that total IgG, unlike IgG4 subclass, positively responded to OvMANE1, strongly suggesting its complementarity to the Ov-16 diagnostic tool, which detects Ov-16 IgG4 antibodies. Overall, OvMANE1 exhibited the potential to be utilized in the development of specific diagnostic tools—based on both antibody capture and antigen capture reactions—which are indispensable to monitor the progress of onchocerciasis elimination programs. View Full-Text
Keywords: Onchocerca volvulus; OvMANE1; chimeric antigen; IgG; diagnosis Onchocerca volvulus; OvMANE1; chimeric antigen; IgG; diagnosis
Show Figures

Figure 1

MDPI and ACS Style

Shintouo, C.M.; Shey, R.A.; Nebangwa, D.N.; K. Esoh, K.; Nongley, N.F.; Nguve, J.E.; Giron, P.; Mutesa, L.; Vanhamme, L.; Souopgui, J.; Ghogomu, S.M.; Njemini, R. In Silico Design and Validation of OvMANE1, a Chimeric Antigen for Human Onchocerciasis Diagnosis. Pathogens 2020, 9, 495.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop