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Characterization of Pathogenic and Nonpathogenic Fusarium oxysporum Isolates Associated with Commercial Tomato Crops in the Andean Region of Colombia
Open AccessArticle

Species-Specific Impact of Fusarium Infection on the Root and Shoot Characteristics of Asparagus

Plant-Microbe Systems, Leibniz Institute of Vegetable and Ornamental Crops (IGZ) e.V., 14979 Großbeeren, Germany
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Pathogens 2020, 9(6), 509; https://doi.org/10.3390/pathogens9060509
Received: 25 March 2020 / Revised: 15 June 2020 / Accepted: 22 June 2020 / Published: 24 June 2020
(This article belongs to the Special Issue Soil-Borne Plant Pathogenic Fungi)
Soil-borne pathogens can have considerable detrimental effects on asparagus (Asparagus officinalis) growth and production, notably caused by the Fusarium species F. oxysporum f.sp. asparagi, F. proliferatum and F. redolens. In this study, their species-specific impact regarding disease severity and root morphological traits was analysed. Additionally, various isolates were characterised based on in vitro physiological activities and on protein extracts using matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS). The response of two asparagus cultivars to the different Fusarium species was evaluated by inoculating experiments. Differences in aggressiveness were observed between Fusarium species and their isolates on roots, while no clear disease symptoms became visible in ferns eight weeks after inoculation. F. redolens isolates Fred1 and Fred2 were the most aggressive strains followed by the moderate aggressive F. proliferatum and the less and almost non-aggressive F. oxysporum isolates, based on the severity of disease symptoms. Fungal DNA in stem bases and a significant induction of pathogenesis-related gene expression was detectable in both asparagus cultivars. A significant negative impact of the pathogens on the root characteristics total root length, volume, and surface area was detected for each isolate tested, with Fred1 causing the strongest effects. No significant differences between the tested asparagus cultivars were observed. View Full-Text
Keywords: Fusarium oxysporum f.sp. asparagi; Fusarium proliferatum; Fusarium redolens; MALDI-TOF MS; morphology imaging; plant pathogen interaction; RT-qPCR; soil-borne fungal pathogens Fusarium oxysporum f.sp. asparagi; Fusarium proliferatum; Fusarium redolens; MALDI-TOF MS; morphology imaging; plant pathogen interaction; RT-qPCR; soil-borne fungal pathogens
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Djalali Farahani-Kofoet, R.; Witzel, K.; Graefe, J.; Grosch, R.; Zrenner, R. Species-Specific Impact of Fusarium Infection on the Root and Shoot Characteristics of Asparagus. Pathogens 2020, 9, 509.

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