Next Article in Journal
Hypermutation as an Evolutionary Mechanism for Achromobacter xylosoxidans in Cystic Fibrosis Lung Infection
Previous Article in Journal
Staphylococcus saprophyticus Proteomic Analyses Elucidate Differences in the Protein Repertories among Clinical Strains Related to Virulence and Persistence
Previous Article in Special Issue
Genetic Diversity of Fusarium oxysporum f. sp. cubense Causing Panama Wilt of Banana in the Philippines
Open AccessArticle

Characterization of Pathogenic and Nonpathogenic Fusarium oxysporum Isolates Associated with Commercial Tomato Crops in the Andean Region of Colombia

1
Corporación Colombiana de Investigación Agropecuaria. AGROSAVIA, Km 14 vía Mosquera-Bogotá, Mosquera 250047, Colombia
2
Departamento de Física y Química, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Colombia sede Manizales, Manizales 170004, Colombia
3
Facultad de Ciencias Agropecuarias, Universidad de Caldas, Manizales 170004, Colombia
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Pathogens 2020, 9(1), 70; https://doi.org/10.3390/pathogens9010070
Received: 17 December 2019 / Revised: 7 January 2020 / Accepted: 15 January 2020 / Published: 20 January 2020
(This article belongs to the Special Issue Soil-Borne Plant Pathogenic Fungi)
In Colombia, tomato production under protected conditions represents an important economic contribution to the agricultural sector. Fusarium wilt diseases, caused by pathogenic formae speciales of the soil-borne fungus Fusarium oxysporum Schltdl., cause significant yield losses in tomatoes throughout the world. Investigation of the F. oxysporum–tomato pathosystem in Colombia is required to develop appropriate alternative disease management. In this study, 120 fungal isolates were obtained from four different departments in the Central Andean Region in Colombia from tomato crops with symptoms of wilt disease. A molecular characterization of the fungal isolates was performed using the SIX1, SIX3, and SIX4 effector genes of Fusarium oxysporum f. sp. lycopersici W.C. Snyder & H.N. Hansen (Fol). Additionally, we developed a new specific marker to distinguish between Fusarium oxysporum f. sp. radicis-lycopersici Jarvis & Shoemaker (Forl) and Fol isolates. Furthermore, a phylogenetic analysis using the Translation Elongation Factor 1-alpha (EF1a) gene was performed with the collected isolates. Two isolates (named Fol59 and Fol-UDC10) were identified as Fol race 2, four isolates were identified as Forl, six isolates were identified as F. solani, and most of the isolates were grouped within the F. oxysporum species complex. The phylogenetic tree of EF1a showed that most of the isolates could potentially correspond to nonpathogenic strains of F. oxysporum. Additional pathogenicity assays carried out with Fol59 and Fol-UDC10 confirmed that both isolates were highly virulent strains. This study represents a contribution to the understanding of the local interaction between tomatoes and F. oxysporum in Colombia. View Full-Text
Keywords: Fusarium wilt; physiological races; SIX genes; virulence; forma specialis; Solanaceae Fusarium wilt; physiological races; SIX genes; virulence; forma specialis; Solanaceae
Show Figures

Figure 1

MDPI and ACS Style

Carmona, S.L.; Burbano-David, D.; Gómez, M.R.; Lopez, W.; Ceballos, N.; Castaño-Zapata, J.; Simbaqueba, J.; Soto-Suárez, M. Characterization of Pathogenic and Nonpathogenic Fusarium oxysporum Isolates Associated with Commercial Tomato Crops in the Andean Region of Colombia. Pathogens 2020, 9, 70.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop