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Proposal of A New Bois Noir Epidemiological Pattern Related to ‘Candidatus Phytoplasma Solani’ Strains Characterized by A Possible Moderate Virulence in Tuscany
Open AccessArticle

Sequence Analysis of New Tuf Molecular Types of ‘Candidatus Phytoplasma Solani’ in Iranian Vineyards

Department of Agricultural, Food and Environmental Sciences, Marche Polytechnic University, 60131 Ancona, Italy
Plant Protection Research Department, Fars Agricultural and Natural Resources Research and Education Centre, AREEO, Zarghan 617-71555, Iran
Author to whom correspondence should be addressed.
Pathogens 2020, 9(6), 508;
Received: 22 April 2020 / Revised: 11 June 2020 / Accepted: 17 June 2020 / Published: 24 June 2020
Grapevine Bois noir (BN) is caused by ‘Candidatus Phytoplasma solani’ (‘Ca. P. solani’) and is one of the most important phytoplasma diseases in the Euro-Mediterranean viticultural areas. The epidemiology of BN can include grapevine as a plant host and is usually transmitted via sap-sucking insects that inhabit herbaceous host plants. Tracking the spread of ‘Ca. P. solani’ strains is of great help for the identification of plant reservoirs and insect vectors involved in local BN outbreaks. The molecular epidemiology of ‘Ca. P. solani’ is primarily based on sequence analysis of the tuf housekeeping gene (which encodes elongation factor Tu). In this study, molecular typing of tuf, through restriction fragment length polymorphism and sequencing, was carried out on grapevine samples from Iranian vineyards. According to the molecular characterization, three molecular types—tuf b1, tuf b5 and tuf b6—were found, with tuf b1 being the most prominent. These data provide further knowledge of tuf gene diversity and question the ecological role of such “minor” tuf types in Iranian vineyards, which have been detected only in grapevines. View Full-Text
Keywords: grapevine; phytoplasma; phytoplasma diseases; stolbur; tuf; Vitis vinifera grapevine; phytoplasma; phytoplasma diseases; stolbur; tuf; Vitis vinifera
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Jamshidi, E.; Murolo, S.; Salehi, M.; Romanazzi, G. Sequence Analysis of New Tuf Molecular Types of ‘Candidatus Phytoplasma Solani’ in Iranian Vineyards. Pathogens 2020, 9, 508.

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