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Open AccessArticle

Long-Term Incubation PrPCWD with Soils Affects Prion Recovery but Not Infectivity

1
Agricultural, Life and Environmental Sciences Faculty, University of Alberta, Edmonton, AB T6G 2G8, Canada
2
Faculty of Science, University of Alberta, Edmonton, AB T6G 2M8, Canada
3
Department of Biology, Carleton University, Ottawa, ON K1S 5B6, Canada
*
Author to whom correspondence should be addressed.
Pathogens 2020, 9(4), 311; https://doi.org/10.3390/pathogens9040311
Received: 10 March 2020 / Revised: 20 April 2020 / Accepted: 21 April 2020 / Published: 23 April 2020
(This article belongs to the Special Issue Prions and Prion-like Transmissible Protein Pathogens)
Chronic wasting disease (CWD) is a contagious prion disease of cervids. The infectious agent is shed from animals at the preclinical and clinical stages of disease where it persists in the environment as a reservoir of CWD infectivity. In this study, we demonstrate that long-term incubation of CWD prions (generated from tg-mice infected with deer or elk prions) with illite, montmorillonite (Mte) and whole soils results in decreased recovery of PrPCWD, suggesting that binding becomes more avid and irreversible with time. This continual decline of immunoblot PrPCWD detection did not correlate with prion infectivity levels. Bioassay showed no significant differences in incubation periods between mice inoculated with 1% CWD brain homogenate (BH) and with the CWD-BH pre-incubated with quartz or Luvisolic Ae horizon for 1 or 30 weeks. After 55 weeks incubation with Chernozem and Luvisol, bound PrPCWD was not detectable by immunoblotting but remained infectious. This study shows that although recovery of PrPCWD bound to soil minerals and whole soils with time become more difficult, prion infectivity is not significantly altered. Detection of prions in soil is, therefore, not only affected by soil type but also by length of time of the prion–soil interaction. View Full-Text
Keywords: prion protein; soil; CWD; prolonged incubation; CWD infectivity; prion detection prion protein; soil; CWD; prolonged incubation; CWD infectivity; prion detection
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Kuznetsova, A.; McKenzie, D.; Cullingham, C.; Aiken, J.M. Long-Term Incubation PrPCWD with Soils Affects Prion Recovery but Not Infectivity. Pathogens 2020, 9, 311.

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