Vertical Profile Characteristics of Dissolved Organic Matter Biochemistry in the Tropical Reservoir Shaped by Hydrodynamic Forces
, Miao Chen 1,2,3,4,5,†, Huiran Liu 2,3,4,5, Han Wang 1,2,3,4,5, Ziyu Ning 1,2,3,4,5, Wen Zhang 1,2,3,4,5, Yuqin Liu 1,2,3,4,5,* and Min Tang 6,*Round 1
Reviewer 1 Report
Comments and Suggestions for AuthorsBefore the text can be accepted, the author may consider the following improvements:
1. Introduction: The state of the art is effectively stated; however, because many studies have been completed in this subject, the author may conduct a gap review to increase the study novelty.
2. The author provides various physicochemical parameters of water samples, however the conclusion does not emphasize the significance of the properties to the main aim of this study.
3. The data was adequately presented, but the commentary lacked depth. Please give any comparisons and explain why the results were as they are.
4. The UV-VIs scanning data was not given.
5. Line 215: Under strong microbial activity, DOM molecules with high humification degrees and complicated structures are converted into smaller compounds: How does the DOM alter with depth?
6. The FTIR and PARAFAC analyses require more in-depth discussion.
7. The conclusion can be improved by including the major point of the discussion in comments 3, 4, and 5.
Author Response
Please see the attachment.
Author Response File: 
Author Response.pdf
Reviewer 2 Report
Comments and Suggestions for Authors-Line 32, when introduces the abbreviation for the first time, authors must give the full description.
-Abstract should be enriched with key numerical finding and operating conditions.
-The introduction section needs to be re-organized to provide details in the importance of this study, advantages of this hydrodynamic force system, specify the scientific issues and research gap with the detailed literature data. What is the novelty of this study and method development?
- Improve the quality of writing.
- The discussion is too general. Improve the discussion section with the detailed scientific manner.
- Reviewer could not find the solid discussion on the role of hydrodynamic forces.
- Similarly, reviewer could not find the solid discussion on the biochemistry-oriented discussion on this manuscript.
- There are many terminologies are introduced without any description and connections.
- when was the samples taken? Will the sampling periods be affecting the components of study?
- Provide the complete raw sample analytical data.
- was there any major weathering on or before the sampling period or area. If YES, will it impact on the sample components.
Author Response
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Author Response File: Author Response.pdf
Reviewer 3 Report
Comments and Suggestions for AuthorsThe article presents a very interesting analysis of changes in DOM content in water across different locations of the reservoir. In my opinion, the article should be supplemented with the following information:
Introduction
The introduction should present the results of similar studies concerning factors influencing the distribution of DOM content, rather than discussing analytical methods used for characterizing NOM. The introduction should be revised accordingly.
Research Methodology
Please provide a detailed description of the methods used to analyze all parameters included in the study results, such as ammonium ion concentration or pH value. The methodology should outline step-by-step the procedures and experimental setup, including:
Water Sampling: How water samples were collected from different depths, the frequency of such analyses, and whether they were repeated multiple times to ensure accuracy.
Analyzed DOM Components: Specify which DOM components were analyzed, the rationale for selecting these components, and their influence or contribution to DOM content.
Experimental Procedures: Detail the methods used for analyzing these components and other parameters.
Explanation of Parameters and Abbreviations: Clearly define all abbreviations and parameters presented in the results, such as E3 and E4, and explain their significance.
This section should provide a clear, comprehensive overview of the experimental design and analytical approaches.
In my opinion, the study should have been repeated multiple times to account for the variability in water composition and the influence of weather factors on DOM distribution. Therefore, I believe that the presented results are insufficient to draw definitive conclusions.
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
Round 2
Reviewer 1 Report
Comments and Suggestions for AuthorsThe authors have address all raised comment. This manuscript can be accepted for publication
Reviewer 2 Report
Comments and Suggestions for AuthorsThe manuscript is well revised.
Reviewer 3 Report
Comments and Suggestions for AuthorsThe authors corrected the article according to my suggestions. In my opinion, the article can be published in this form.
