Effects of Probiotics on the Water Quality, Growth Performance, Immunity, Digestion, and Intestinal Flora of Giant Freshwater Prawn (Macrobrachium rosenbergii) in the Biofloc Culture System
, Qiyou Xu 1,2, Shenghao Li 1,2, Dakua Zheng 1,2, Rongfei Zhang 1,2, Jianhua Zhao 1,2,* and Ting Wang 3,*Round 1
Reviewer 1 Report
The paper presents an interesting research work. In general, the paper is properly organized. However, some of description needs to be explained more in detail. I think that the current paper could be accepted after revision.
My criticism/ recommendation to the manuscript:
- The text should be edited by a native English person.
- Upgrade the introduction. Briefly state similar works that used of biofloc culture system
-What is suitable method for disposal biofloc culture system? Add disposal method of after removal process.
-Finally, it is recommended to add the all references in your paper
- English needs to be carefully checked and polished by a native English speaker
Author Response
1.The text should be edited by a native English person.
Reply：We invited native English speakers to polish and modify the article.
2.Upgrade the introduction. Briefly state similar works that used of biofloc culture system.
Reply：Combining the opinions of the two reviewers on the introduction part, the introduction part is deleted and reordered to make the readers understand more clearly.
3.What is suitable method for disposal biofloc culture system? Add disposal method of after removal process.
-Finally, it is recommended to add the all references in your paper
Reply：My understanding of this sentence.
In the actual production of aquaculture, there may be a phenomenon of excessive biofloc. It is necessary to manually salvage biofloc to prevent excessive biofloc in water.
However, there was no excessive amount of biofloc in this experiment. Macrobrachium rosenbergii had obvious feeding behavior and reduced the amount of biofloc in water.
4.English needs to be carefully checked and polished by a native English speaker
Reply：We invited native English speakers to polish and modify the article.
Reviewer 2 Report
I find this article very interesting. In order to explore the effects of probiotics on the water quality, growth performance, non-specific immunity, digestion, and intestinal flora of Macrobrachium rosenbergii in the biofloc culture system carried out an interesting experiment in which it was proved that the addition of Bacillus subtilis and effective microorganisms in the biofloc culture system had a positive impact on the water body, immunity, and digestion of Macrobrachium rosenbergii. In addition, the addition of Bacillus in the biofloc could increase the abundance of beneficial bacteria in the intestinal tract of Macrobrachium rosenbergii. It is very valuable for this article. In general, the manuscript is well organized, although I have a few remarks here. The wording of the problems agrees with the title and is eloquent. The methods used to collect data in this article are clearly explained with the small exception mentioned below. The quality of citations is good , the authors refer to interesting works in this field of research. The topic is interesting and the results are concrete and useful to the scientific community. However, I believe some adjustments need to be made:
1. Please do not repeat the same entry in the conclusion as in the abstract.
2. In my opinion, the introduction requires ordering, reorganization, so that the reader is clearly introduced to the subject of the implemented issues.
3. Please explain how the water temperature, pH and dissolved oxygen were measured. Was it one measurement in one place or several measurements in different places? It is worth explaining it or presenting it on a diagram. Were there any changes in this area during the experiment?
4. In lines 346 - 359, format the text.
The publication is a valuable source of information and forms the basis for further analyzes and implementation of new solutions.
Thank you for considering my opinion. I encourage the authors to continue working on improving the manuscript.
Author Response
1.Please do not repeat the same entry in the conclusion as in the abstract.
Reply：We have revised it to " Overall, adding probiotics affected water quality, Macrobrachium rosenbergii performance, and microbial community. The results showed that Bacillus subtilis was a good biofloc additive. "
2.In my opinion, the introduction requires ordering, reorganization, so that the reader is clearly introduced to the subject of the implemented issues.
Reply：Combining the opinions of the two reviewers on the introduction part, the introduction part is deleted and reordered to make the readers understand more clearly.
3.Please explain how the water temperature, pH and dissolved oxygen were measured. Was it one measurement in one place or several measurements in different places? It is worth explaining it or presenting it on a diagram. Were there any changes in this area during the experiment?
Reply：The water quality measurement process has been added to the material and method positions.
In addition, there was no significant difference in pH, temperature and dissolved oxygen between the groups during the whole stage of the experiment. It is a bit abrupt to add pictures or tables to the article, so we describe the aquaculture water environment in the experimental stage with words in the material and method position of the article.
4.In lines 346 - 359, format the text.
Reply：We have modified its format.
