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Article

Haplotype Structures and Protein Levels of TGFB1 in HPV Infection and Cervical Lesion: A Case-Control Study

by
Kleber Paiva Trugilo
1,
Guilherme Cesar Martelossi Cebinelli
1,
Érica Romão Pereira
1,2,
Nádia Calvo Martins Okuyama
1,2,
Fernando Cezar-dos-Santos
1,
Eliza Pizarro Castilha
1,
Tamires Flauzino
3,
Valéria Bumiller-Bini Hoch
4,
Maria Angelica Ehara Watanabe
5,
Roberta Losi Guembarovski
2 and
Karen Brajão de Oliveira
1,*
1
Laboratory of Molecular Genetics and Immunology, Department of Pathological Sciences, Biological Sciences Center, State University of Londrina, Londrina 86057-970, PR, Brazil
2
Laboratory of Mutagenesis and Oncogenetics, Department of Biological Sciences, State University of Londrina, Londrina 86057-970, PR, Brazil
3
Laboratory of Research in Applied Immunology, State University of Londrina, Londrina 86057-970, PR, Brazil
4
Laboratory of Human Molecular Genetics, Department of Genetics, Federal University of Paraná, Curitiba 80060-000, PR, Brazil
5
Laboratory of Studies and Applications of DNA Polymorphisms and Immunology, Department of Pathological Sciences, Biological Sciences Center, State University of Londrina, Londrina 86057-970, PR, Brazil
*
Author to whom correspondence should be addressed.
Cells 2023, 12(1), 84; https://doi.org/10.3390/cells12010084
Submission received: 24 November 2022 / Revised: 17 December 2022 / Accepted: 21 December 2022 / Published: 25 December 2022
(This article belongs to the Special Issue Molecular and Immunological Mechanisms of HPV-Induced Carcinogenesis)
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Abstract

This study aimed to verify the role of TGFB1 variants (c.–1638G>A, c.–1347C>T, c.29C>T, and c.74G>C) in HPV infection susceptibility and cervical lesions development, and their impact on TGFB1 cervical and plasma levels. TGFB1 genotypes were assessed with PCR-RFLP and haplotypes were inferred for 190 HPV-uninfected and 161 HPV-infected women. TGFB1 levels were determined with immunofluorimetric assay. Case-control analyses were performed with logistic regression adjusted for possible confounders. Women carrying –1347TT or –1347CT+TT as well as those with 29CT, 29CC, or 29CT+CC were more likely to have HPV than –1347CC and 29TT carriers, respectively. Regarding haplotypes, the most frequent were *4 (GCTG) and *3 (GTCG). Women *4/*4 were less likely to have HPV than those with no *4 copy. Comparing the inheritance of *3 and *4, carriers of *3/*4 or *3/*3 were more susceptible to HPV than *4/*4. The TGFB1 plasma and cervical levels were higher in the infected patients. Plasma levels were also higher in infected women with low-grade lesions. HPV-infected patients carrying *3/Other and *3/Other+*3/*3 presented lower TGFB1 plasma levels than those with no copy of *3. TGFB1 variants could contribute to the comprehension of the TGFB1 role in HPV-caused cervical disease.
Keywords: polymorphism; rs1800468; rs1800469; rs1800470; rs1800471 polymorphism; rs1800468; rs1800469; rs1800470; rs1800471
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MDPI and ACS Style

Trugilo, K.P.; Cebinelli, G.C.M.; Pereira, É.R.; Okuyama, N.C.M.; Cezar-dos-Santos, F.; Castilha, E.P.; Flauzino, T.; Hoch, V.B.-B.; Watanabe, M.A.E.; Guembarovski, R.L.; et al. Haplotype Structures and Protein Levels of TGFB1 in HPV Infection and Cervical Lesion: A Case-Control Study. Cells 2023, 12, 84. https://doi.org/10.3390/cells12010084

AMA Style

Trugilo KP, Cebinelli GCM, Pereira ÉR, Okuyama NCM, Cezar-dos-Santos F, Castilha EP, Flauzino T, Hoch VB-B, Watanabe MAE, Guembarovski RL, et al. Haplotype Structures and Protein Levels of TGFB1 in HPV Infection and Cervical Lesion: A Case-Control Study. Cells. 2023; 12(1):84. https://doi.org/10.3390/cells12010084

Chicago/Turabian Style

Trugilo, Kleber Paiva, Guilherme Cesar Martelossi Cebinelli, Érica Romão Pereira, Nádia Calvo Martins Okuyama, Fernando Cezar-dos-Santos, Eliza Pizarro Castilha, Tamires Flauzino, Valéria Bumiller-Bini Hoch, Maria Angelica Ehara Watanabe, Roberta Losi Guembarovski, and et al. 2023. "Haplotype Structures and Protein Levels of TGFB1 in HPV Infection and Cervical Lesion: A Case-Control Study" Cells 12, no. 1: 84. https://doi.org/10.3390/cells12010084

APA Style

Trugilo, K. P., Cebinelli, G. C. M., Pereira, É. R., Okuyama, N. C. M., Cezar-dos-Santos, F., Castilha, E. P., Flauzino, T., Hoch, V. B.-B., Watanabe, M. A. E., Guembarovski, R. L., & de Oliveira, K. B. (2023). Haplotype Structures and Protein Levels of TGFB1 in HPV Infection and Cervical Lesion: A Case-Control Study. Cells, 12(1), 84. https://doi.org/10.3390/cells12010084

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