Genetically Modified M13 Bacteriophage Nanonets for Enzyme Catalysis and Recovery
Abstract
:1. Introduction
2. Results and Discussion
2.1. Biotinylation and Characterization of M13 Bacteriophages
2.2. Nanonet Synthesis and Characterization
2.3. Enzyme Activity Assays of Nanonets
2.4. Syringe Filter Nanonet Reactor
3. Materials and Methods
3.1. Materials
3.2. M13 Bacteriophage Biotinylation
3.3. Characterization of Biotinylation Yield of M13 Bacteriophage
3.4. M13 Bacteriophage Nanonet
3.5. Sulfo-SIAB Cross-Linked Urease Nanonet (Enzyme-Nanonets)
3.6. Glutaraldehyde Cross-Linked Urease Nanonet
3.7. Fluorescence Imaging
3.8. Urease Activity Assays
3.9. Syringe Filter Reactor
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Kadiri, V.M.; Alarcón-Correa, M.; Ruppert, J.; Günther, J.-P.; Bill, J.; Rothenstein, D.; Fischer, P. Genetically Modified M13 Bacteriophage Nanonets for Enzyme Catalysis and Recovery. Catalysts 2019, 9, 723. https://doi.org/10.3390/catal9090723
Kadiri VM, Alarcón-Correa M, Ruppert J, Günther J-P, Bill J, Rothenstein D, Fischer P. Genetically Modified M13 Bacteriophage Nanonets for Enzyme Catalysis and Recovery. Catalysts. 2019; 9(9):723. https://doi.org/10.3390/catal9090723
Chicago/Turabian StyleKadiri, Vincent Mauricio, Mariana Alarcón-Correa, Jacqueline Ruppert, Jan-Philipp Günther, Joachim Bill, Dirk Rothenstein, and Peer Fischer. 2019. "Genetically Modified M13 Bacteriophage Nanonets for Enzyme Catalysis and Recovery" Catalysts 9, no. 9: 723. https://doi.org/10.3390/catal9090723