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Open AccessArticle
Peer-Review Record

Genetically Modified M13 Bacteriophage Nanonets for Enzyme Catalysis and Recovery

Catalysts 2019, 9(9), 723; https://doi.org/10.3390/catal9090723
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Catalysts 2019, 9(9), 723; https://doi.org/10.3390/catal9090723
Received: 8 August 2019 / Accepted: 23 August 2019 / Published: 27 August 2019
(This article belongs to the Special Issue State of the Art and Future Trends in Nanostructured Biocatalysis)

Round 1

Reviewer 1 Report

The manuscript by Kadiri et al describes the preparation and usage of phages nanonets for enzyme catalysis. This work builds on their previous work, where they immobilized phages on magnetic particles, however in this work M13 bacteriophages were modified with AviTag and crosslinked with streptavidin to form nanonets. After functionalizing the nanonets with urease, the authors showed that the catalysts can be easily recovered, and it retains its activity through several cycles of reactions and also over time. They also a built a continuous flow-through device by depositing the enzyme nanonets on a syringe filter.

The paper is well structured and clearly written and the necessary control experiments were presented. Therefore, the paper can be published in the presented form. 

Reviewer 2 Report

In this report, authors showed that immobilization to  M13 bacteriophages ensured high enzyme activity and  simple recovery of the enzymes. I enjoyed reading this manucript and recommends this for the publication in this journal.

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