Genetically Modified M13 Bacteriophage Nanonets for Enzyme Catalysis and Recovery
, Mariana Alarcón-Correa 1, Jacqueline Ruppert 1,2, Jan-Philipp Günther 1,2, Joachim Bill 3, Dirk Rothenstein 3 and Peer Fischer 1,2,*Round 1
Reviewer 1 Report
The manuscript by Kadiri et al describes the preparation and usage of phages nanonets for enzyme catalysis. This work builds on their previous work, where they immobilized phages on magnetic particles, however in this work M13 bacteriophages were modified with AviTag and crosslinked with streptavidin to form nanonets. After functionalizing the nanonets with urease, the authors showed that the catalysts can be easily recovered, and it retains its activity through several cycles of reactions and also over time. They also a built a continuous flow-through device by depositing the enzyme nanonets on a syringe filter.
The paper is well structured and clearly written and the necessary control experiments were presented. Therefore, the paper can be published in the presented form.
Reviewer 2 Report
In this report, authors showed that immobilization to M13 bacteriophages ensured high enzyme activity and simple recovery of the enzymes. I enjoyed reading this manucript and recommends this for the publication in this journal.
