Next Article in Journal
Enhanced Selective Production of Arenes and Regenerating Rate in Aryl Ether Hydrogenolysis over Mesoporous Nickel in Plug-Flow Reactors
Previous Article in Journal
Y-Modified MCM-22 Supported PdOx Nanocrystal Catalysts for Catalytic Oxidation of Toluene
Previous Article in Special Issue
Genetically Modified M13 Bacteriophage Nanonets for Enzyme Catalysis and Recovery
Open AccessArticle

Peptide–Gold Nanoparticle Conjugates as Artificial Carbonic Anhydrase Mimics

Department of Biology, Chemistry and Pharmacy, Institute of Chemistry and Biochemistry, Freie Universität Berlin, 14195 Berlin, Germany
*
Author to whom correspondence should be addressed.
Catalysts 2019, 9(11), 903; https://doi.org/10.3390/catal9110903
Received: 24 September 2019 / Revised: 21 October 2019 / Accepted: 27 October 2019 / Published: 29 October 2019
(This article belongs to the Special Issue State of the Art and Future Trends in Nanostructured Biocatalysis)
We herein describe the design and synthesis of a catalytically active peptide–gold nanoparticle conjugate (Pep-Au-NP) that binds Zn(II) within its peptide monolayer and develops carbonic anhydrase activity. Specifically, a modified variant of the β-sheet forming IHIHIQI-peptide (IHQ), which forms an interstrand 3-His Zn(II)-binding site, was used as a ligand for spherical gold nanoparticles (Au-NPs). The resulting immobilized peptide maintains its ability to form β-sheets, as determined by circular dichroism (CD)-spectroscopy and, thus, maintains its ability to form Zn(II)-binding sites. The addition of Zn(II)-ions to the peptide–gold nanoparticle conjugates ([email protected]) resulted in significant improvements in rates of ester hydrolysis of 4-nitrophenyl acetate (4-NPA) and the hydration of CO2 compared to the unconjugated peptide variants. Recycling of the catalyst revealed that [email protected] remains intact with at least 94% of its initial activity after five rounds of CO2 hydration. The herein reported results reveal that Pep-Au-NPs are able to perform reactions catalyzed by natural metalloenzymes and open up new possibilities for the implementation of these conjugates. View Full-Text
Keywords: catalytically active peptides; gold nanoparticle; carbonic anhydrase mimic; artificial enzymes; catalysis catalytically active peptides; gold nanoparticle; carbonic anhydrase mimic; artificial enzymes; catalysis
Show Figures

Figure 1

MDPI and ACS Style

Mikolajczak, D.J.; Koksch, B. Peptide–Gold Nanoparticle Conjugates as Artificial Carbonic Anhydrase Mimics. Catalysts 2019, 9, 903.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop