Next Article in Journal
Efficient and Precise Grinding of Sapphire Glass Based on Dry Electrical Discharge Dressed Coarse Diamond Grinding Wheel
Previous Article in Journal
Simulation and Experiment on Droplet Volume for the Needle-Type Piezoelectric Jetting Dispenser
Open AccessArticle

A Complete Protocol for Rapid and Low-Cost Fabrication of Polymer Microfluidic Chips Containing Three-Dimensional Microstructures Used in Point-of-Care Devices

1
Department of Biotechnology and Biomedicine, Technical University of Denmark, DK 2800 Kgs. Lyngby, Denmark
2
Laboratory of Applied Micro and Nanotechnology (LAMINATE), Division of Microbiology and Production, National Food Institute, Technical University of Denmark, Kemitorvet, Building 204, DK 2800 Lyngby, Denmark
*
Authors to whom correspondence should be addressed.
Micromachines 2019, 10(9), 624; https://doi.org/10.3390/mi10090624
Received: 3 September 2019 / Revised: 12 September 2019 / Accepted: 16 September 2019 / Published: 19 September 2019
(This article belongs to the Special Issue Micro-, Nano-fluidics and Biosensors in Food Safety Applications)
This protocol provides insights into the rapid, low-cost, and largescale fabrication of polymer microfluidic chips containing three-dimensional microstructures used in point-of-care devices for applications such as detection of pathogens via molecular diagnostic methods. The details of the fabrication methods are described in this paper. This study offers suggestions for researchers and experimentalists, both at university laboratories and in industrial companies, to prevent doom fabrication issues. For a demonstration of bio-application in point-of-care testing, the 3D microarrays fabricated are then employed in multiplexed detection of Salmonella (Salmonella Typhimurium and Salmonella Enteritidis), based on a molecular detection technique called solid-phase polymerase chain reaction (SP-PCR). View Full-Text
Keywords: polymer; microfluidic chips; point of care; three-dimensional microstructures; protocol polymer; microfluidic chips; point of care; three-dimensional microstructures; protocol
Show Figures

Figure 1

MDPI and ACS Style

Nguyen, T.; Chidambara Vinayaka, A.; Duong Bang, D.; Wolff, A. A Complete Protocol for Rapid and Low-Cost Fabrication of Polymer Microfluidic Chips Containing Three-Dimensional Microstructures Used in Point-of-Care Devices. Micromachines 2019, 10, 624.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop