Effect of Cyclic Stretch on Tissue Maturation in Myoblast-Laden Hydrogel Fibers

Bansai, Shinako; Morikura, Takashi; Onoe, Hiroaki; Miyata, Shogo

doi:10.3390/mi10060399

Open AccessArticle

Effect of Cyclic Stretch on Tissue Maturation in Myoblast-Laden Hydrogel Fibers

¹

Graduate School of Science and Technology, Keio University, 3-14-1 Hiyoshi, Yokohama 223-8522, Japan

²

Department of Mechanical Engineering, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Yokohama 223-8522, Japan

^*

Author to whom correspondence should be addressed.

^†

These authors have equally contributed to this work.

Micromachines 2019, 10(6), 399; https://doi.org/10.3390/mi10060399

Submission received: 17 May 2019 / Revised: 9 June 2019 / Accepted: 13 June 2019 / Published: 15 June 2019

(This article belongs to the Special Issue Selected Papers from the 9th Symposium on Micro-Nano Science and Technology on Micromachines)

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Abstract

Engineering of the skeletal muscles has attracted attention for the restoration of damaged muscles from myopathy, injury, and extraction of malignant tumors. Reconstructing a three-dimensional muscle using living cells could be a promising approach. However, the regenerated tissue exhibits a weak construction force due to the insufficient tissue maturation. The purpose of this study is to establish the reconstruction system for the skeletal muscle. We used a cell-laden core-shell hydrogel microfiber as a three-dimensional culture to control the cellular orientation. Moreover, to mature the muscle tissue in the microfiber, we also developed a custom-made culture device for imposing cyclic stretch stimulation using a motorized stage and the fiber-grab system. As a result, the directions of the myotubes were oriented and the mature myotubes could be formed by cyclic stretch stimulation.

Keywords: myoblast; skeletal muscle; core-shell hydrogel fiber; cyclic stretch; engineered muscle

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MDPI and ACS Style

Bansai, S.; Morikura, T.; Onoe, H.; Miyata, S. Effect of Cyclic Stretch on Tissue Maturation in Myoblast-Laden Hydrogel Fibers. Micromachines 2019, 10, 399. https://doi.org/10.3390/mi10060399

AMA Style

Bansai S, Morikura T, Onoe H, Miyata S. Effect of Cyclic Stretch on Tissue Maturation in Myoblast-Laden Hydrogel Fibers. Micromachines. 2019; 10(6):399. https://doi.org/10.3390/mi10060399

Chicago/Turabian Style

Bansai, Shinako, Takashi Morikura, Hiroaki Onoe, and Shogo Miyata. 2019. "Effect of Cyclic Stretch on Tissue Maturation in Myoblast-Laden Hydrogel Fibers" Micromachines 10, no. 6: 399. https://doi.org/10.3390/mi10060399

APA Style

Bansai, S., Morikura, T., Onoe, H., & Miyata, S. (2019). Effect of Cyclic Stretch on Tissue Maturation in Myoblast-Laden Hydrogel Fibers. Micromachines, 10(6), 399. https://doi.org/10.3390/mi10060399

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

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Effect of Cyclic Stretch on Tissue Maturation in Myoblast-Laden Hydrogel Fibers

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