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Article

Study Effects of Drug Treatment and Physiological Physical Stimulation on Surfactant Protein Expression of Lung Epithelial Cells Using a Biomimetic Microfluidic Cell Culture Device

1
Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan
2
College of Engineering, Chang Gung University, Taoyuan 33302, Taiwan
*
Author to whom correspondence should be addressed.
Micromachines 2019, 10(6), 400; https://doi.org/10.3390/mi10060400
Received: 10 May 2019 / Revised: 4 June 2019 / Accepted: 14 June 2019 / Published: 16 June 2019
(This article belongs to the Special Issue Organs-on-chips)
This paper reports a biomimetic microfluidic device capable of reconstituting physiological physical microenvironments in lungs during fetal development for cell culture. The device integrates controllability of both hydrostatic pressure and cyclic substrate deformation within a single chip to better mimic the in vivo microenvironments. For demonstration, the effects of drug treatment and physical stimulations on surfactant protein C (SPC) expression of lung epithelial cells (A549) are studied using the device. The experimental results confirm the device’s capability of mimicking in vivo microenvironments with multiple physical stimulations for cell culture applications. Furthermore, the results indicate the critical roles of physical stimulations in regulating cellular behaviors. With the demonstrated functionalities and performance, the device is expected to provide a powerful tool for further lung development studies that can be translated to clinical observation in a more straightforward manner. Consequently, the device is promising for construction of more in vitro physiological microenvironments integrating multiple physical stimulations to better study organ development and its functions. View Full-Text
Keywords: microfluidic device; cell culture; organ-on-chips; lung epithelial cell; surfactant protein microfluidic device; cell culture; organ-on-chips; lung epithelial cell; surfactant protein
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MDPI and ACS Style

Lin, T.-R.; Yeh, S.-L.; Peng, C.-C.; Liao, W.-H.; Tung, Y.-C. Study Effects of Drug Treatment and Physiological Physical Stimulation on Surfactant Protein Expression of Lung Epithelial Cells Using a Biomimetic Microfluidic Cell Culture Device. Micromachines 2019, 10, 400. https://doi.org/10.3390/mi10060400

AMA Style

Lin T-R, Yeh S-L, Peng C-C, Liao W-H, Tung Y-C. Study Effects of Drug Treatment and Physiological Physical Stimulation on Surfactant Protein Expression of Lung Epithelial Cells Using a Biomimetic Microfluidic Cell Culture Device. Micromachines. 2019; 10(6):400. https://doi.org/10.3390/mi10060400

Chicago/Turabian Style

Lin, Ting-Ru, Sih-Ling Yeh, Chien-Chung Peng, Wei-Hao Liao, and Yi-Chung Tung. 2019. "Study Effects of Drug Treatment and Physiological Physical Stimulation on Surfactant Protein Expression of Lung Epithelial Cells Using a Biomimetic Microfluidic Cell Culture Device" Micromachines 10, no. 6: 400. https://doi.org/10.3390/mi10060400

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