Bergamot Polyphenol Extract Reduces Hepatocyte Neutral Fat by Increasing Beta-Oxidation
Abstract
:1. Introduction
2. Materials and Methods
2.1. D and 3D Cell Culture
2.2. Treatments
2.3. Cell Viability Assay
2.4. Quantification of Intracellular Neutral Lipid Content
2.5. Lipid Assay
2.6. Real Time-PCR
2.7. Statistical Analysis
3. Results
3.1. Incubation with Bergamot PF Extract Decreases Intracellular Triglycerides in McA Rh7777
3.2. Bergamot PF Extract Increases Expression Levels of Gene of ß-Oxidation and Lipophagy in McA Rh7777
3.3. Bergamot PF Extract Incubation Reduces Intracellular Neutral Lipid Content and Increases Beta-Oxidation in 3D HEPG2/LX2 Spheroids
3.4. Bergamot PF Extract Incubation Reduces Intracellular Neutral Lipid Content in Human Liver Organoid
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Mirarchi, A.; Mare, R.; Musolino, V.; Nucera, S.; Mollace, V.; Pujia, A.; Montalcini, T.; Romeo, S.; Maurotti, S. Bergamot Polyphenol Extract Reduces Hepatocyte Neutral Fat by Increasing Beta-Oxidation. Nutrients 2022, 14, 3434. https://doi.org/10.3390/nu14163434
Mirarchi A, Mare R, Musolino V, Nucera S, Mollace V, Pujia A, Montalcini T, Romeo S, Maurotti S. Bergamot Polyphenol Extract Reduces Hepatocyte Neutral Fat by Increasing Beta-Oxidation. Nutrients. 2022; 14(16):3434. https://doi.org/10.3390/nu14163434
Chicago/Turabian StyleMirarchi, Angela, Rosario Mare, Vincenzo Musolino, Saverio Nucera, Vincenzo Mollace, Arturo Pujia, Tiziana Montalcini, Stefano Romeo, and Samantha Maurotti. 2022. "Bergamot Polyphenol Extract Reduces Hepatocyte Neutral Fat by Increasing Beta-Oxidation" Nutrients 14, no. 16: 3434. https://doi.org/10.3390/nu14163434
APA StyleMirarchi, A., Mare, R., Musolino, V., Nucera, S., Mollace, V., Pujia, A., Montalcini, T., Romeo, S., & Maurotti, S. (2022). Bergamot Polyphenol Extract Reduces Hepatocyte Neutral Fat by Increasing Beta-Oxidation. Nutrients, 14(16), 3434. https://doi.org/10.3390/nu14163434