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Open AccessArticle

The Effect of Isoleucine Supplementation on Body Weight Gain and Blood Glucose Response in Lean and Obese Mice

1
Adelaide Medical School, University of Adelaide, Adelaide, SA 5005, Australia
2
Nutrition, Diabetes and Gut Health, Lifelong Health Theme, South Australian Health and Medical Research Institute (SAHMRI), Adelaide, SA 5001, Australia
3
School of Agriculture, Food and Wine, The University of Adelaide, Glen Osmond, SA 5064, Australia
4
Centre for Community Health Studies, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, Malaysia
5
College of Medicine and Public Health, Flinders Medical Centre, Flinders University, Bedford Park, SA 5042, Australia
6
Women’s and Children’s Hospital, North Adelaide, SA 5006, Australia
7
Clinical Trial Unit, Department of Clinical Research, University of Basel and University Hospital Basel, 4031 Basel, Switzerland
*
Author to whom correspondence should be addressed.
Nutrients 2020, 12(8), 2446; https://doi.org/10.3390/nu12082446
Received: 13 July 2020 / Revised: 6 August 2020 / Accepted: 12 August 2020 / Published: 14 August 2020
(This article belongs to the Special Issue Appetite and Satiety Control-Gut Mechanisms)
Chronic isoleucine supplementation prevents diet-induced weight gain in rodents. Acute-isoleucine administration improves glucose tolerance in rodents and reduces postprandial glucose levels in humans. However, the effect of chronic-isoleucine supplementation on body weight and glucose tolerance in obesity is unknown. This study aimed to investigate the impact of chronic isoleucine on body weight gain and glucose tolerance in lean and high-fat-diet (HFD) induced-obese mice. Male C57BL/6-mice, fed a standard-laboratory-diet (SLD) or HFD for 12 weeks, were randomly allocated to: (1) Control: Drinking water; (2) Acute: Drinking water with a gavage of isoleucine (300 mg/kg) prior to the oral-glucose-tolerance-test (OGTT) or gastric-emptying-breath-test (GEBT); (3) Chronic: Drinking water with 1.5% isoleucine, for a further six weeks. At 16 weeks, an OGTT and GEBT was performed and at 17 weeks metabolic monitoring. In SLD- and HFD-mice, there was no difference in body weight, fat mass, and plasma lipid profiles between isoleucine treatment groups. Acute-isoleucine did not improve glucose tolerance in SLD- or HFD-mice. Chronic-isoleucine impaired glucose tolerance in SLD-mice. There was no difference in gastric emptying between any groups. Chronic-isoleucine did not alter energy intake, energy expenditure, or respiratory quotient in SLD- or HFD-mice. In conclusion, chronic isoleucine supplementation may not be an effective treatment for obesity or glucose intolerance. View Full-Text
Keywords: obesity; amino acid; isoleucine; chronic supplementation; energy expenditure; oral glucose tolerance test; glycaemic control; gastric emptying breath test obesity; amino acid; isoleucine; chronic supplementation; energy expenditure; oral glucose tolerance test; glycaemic control; gastric emptying breath test
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MDPI and ACS Style

O’Rielly, R.; Li, H.; Lim, S.M.; Yazbeck, R.; Kritas, S.; Ullrich, S.S.; Feinle-Bisset, C.; Heilbronn, L.; Page, A.J. The Effect of Isoleucine Supplementation on Body Weight Gain and Blood Glucose Response in Lean and Obese Mice. Nutrients 2020, 12, 2446.

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