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Article

Assessing the Intestinal Permeability and Anti-Inflammatory Potential of Sesquiterpene Lactones from Chicory

1
Instituto de Biologia Experimental e Tecnológica (iBET), Av. República, Qta. Marquês, 2780-157 Oeiras, Portugal
2
CEDOC, Chronic Diseases Research Centre, NOVA Medical School, Faculdade de Ciências Médicas, Universidade NOVA de Lisboa, Campo dos Mártires da Pátria, 130, 1169-056 Lisboa, Portugal
3
Plant Biochemistry and Food Quality Group, Environmental and Biochemical Sciences, The James Hutton Institute, Dundee DD2 5DA, UK
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Nutrients 2020, 12(11), 3547; https://doi.org/10.3390/nu12113547
Received: 7 October 2020 / Revised: 12 November 2020 / Accepted: 16 November 2020 / Published: 19 November 2020
Cichorium intybus L. has recently gained major attention due to large quantities of health-promoting compounds in its roots, such as inulin and sesquiterpene lactones (SLs). Chicory is the main dietary source of SLs, which have underexplored bioactive potential. In this study, we assessed the capacity of SLs to permeate the intestinal barrier to become physiologically available, using in silico predictions and in vitro studies with the well-established cell model of the human intestinal mucosa (differentiated Caco-2 cells). The potential of SLs to modulate inflammatory responses through modulation of the nuclear factor of activated T-cells (NFAT) pathway was also evaluated, using a yeast reporter system. Lactucopicrin was revealed as the most permeable chicory SL in the intestinal barrier model, but it had low anti-inflammatory potential. The SL with the highest anti-inflammatory potential was 11β,13-dihydrolactucin, which inhibited up to 54% of Calcineurin-responsive zinc finger (Crz1) activation, concomitantly with the impairment of the nuclear accumulation of Crz1, the yeast orthologue of human NFAT. View Full-Text
Keywords: calcineurin; NFAT; 11β,13-dihydrolactucin; lactucin; lactucopicrin; 11β,13-dihydrolactucopicrin calcineurin; NFAT; 11β,13-dihydrolactucin; lactucin; lactucopicrin; 11β,13-dihydrolactucopicrin
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MDPI and ACS Style

Matos, M.S.; Anastácio, J.D.; Allwood, J.W.; Carregosa, D.; Marques, D.; Sungurtas, J.; McDougall, G.J.; Menezes, R.; Matias, A.A.; Stewart, D.; Santos, C.N.d. Assessing the Intestinal Permeability and Anti-Inflammatory Potential of Sesquiterpene Lactones from Chicory. Nutrients 2020, 12, 3547. https://doi.org/10.3390/nu12113547

AMA Style

Matos MS, Anastácio JD, Allwood JW, Carregosa D, Marques D, Sungurtas J, McDougall GJ, Menezes R, Matias AA, Stewart D, Santos CNd. Assessing the Intestinal Permeability and Anti-Inflammatory Potential of Sesquiterpene Lactones from Chicory. Nutrients. 2020; 12(11):3547. https://doi.org/10.3390/nu12113547

Chicago/Turabian Style

Matos, Melanie S., José D. Anastácio, J. W. Allwood, Diogo Carregosa, Daniela Marques, Julie Sungurtas, Gordon J. McDougall, Regina Menezes, Ana A. Matias, Derek Stewart, and Cláudia N.d. Santos 2020. "Assessing the Intestinal Permeability and Anti-Inflammatory Potential of Sesquiterpene Lactones from Chicory" Nutrients 12, no. 11: 3547. https://doi.org/10.3390/nu12113547

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