Optimized Extraction by Response Surface Methodology Used for the Characterization and Quantification of Phenolic Compounds in Whole Red Grapes (Vitis vinifera)
by
Lisard Iglesias-Carres 1, Anna Mas-Capdevila 1, Lucía Sancho-Pardo 1, Francisca Isabel Bravo 1
, Miquel Mulero 1, Begoña Muguerza 1,2,* and Anna Arola-Arnal 1
Lisard Iglesias-Carres 1, Anna Mas-Capdevila 1, Lucía Sancho-Pardo 1, Francisca Isabel Bravo 1, Miquel Mulero 1, Begoña Muguerza 1,2,* and Anna Arola-Arnal 1
1
Nutrigenomics Research Group, Departament de Bioquímica i Biotecnologia, Universitat Rovira i Virgili, 43007 Tarragona, Spain
2
EURECAT, Centre Tecnològic de Catalunya, Unit of nutrition and Health, 43204 Reus, Spain
*
Author to whom correspondence should be addressed.
Nutrients 2018, 10(12), 1931; https://doi.org/10.3390/nu10121931
Received: 3 October 2018 / Revised: 17 November 2018 / Accepted: 3 December 2018 / Published: 5 December 2018
(This article belongs to the Special Issue Plant Food, Nutrition and Human Health)
Scientific research has focused on the characterization of bioactive polyphenols from grape seeds and skins, and the pulp has often been overlooked. However, since the beneficial properties of grapes are associated with the consumption of whole fruit, a full extraction and posterior characterization of the phenolic compounds in whole grapes is required to identify the involved bioactive compounds. Such methodologies are not currently available for the whole edible parts of red grapes. This study aimed to determine the best polyphenol extraction conditions of whole red grapes, and apply the method to characterize and quantify the polyphenol composition of three different grapes. The optimized conditions were 80 mL/g, 65% methanol (1% formic acid), 72 °C, and 100 min under agitation of 500 rpm. Also, methanol and ethanol were compared as extraction solvents, and methanol achieved statistically higher extraction rates for anthocyanins. The results of this work suggest a higher quantification of phenolic compounds when red grapes are analyzed whole, including the seeds, pulp, and skin.
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MDPI and ACS Style
Iglesias-Carres, L.; Mas-Capdevila, A.; Sancho-Pardo, L.; Bravo, F.I.; Mulero, M.; Muguerza, B.; Arola-Arnal, A. Optimized Extraction by Response Surface Methodology Used for the Characterization and Quantification of Phenolic Compounds in Whole Red Grapes (Vitis vinifera). Nutrients 2018, 10, 1931.
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