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Toll-Like Receptor 3 Is Involved in Detection of Enterovirus A71 Infection and Targeted by Viral 2A Protease
Article

Viperin Inhibits Enterovirus A71 Replication by Interacting with Viral 2C Protein

CAS Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
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Author to whom correspondence should be addressed.
Viruses 2019, 11(1), 13; https://doi.org/10.3390/v11010013
Received: 13 November 2018 / Revised: 21 December 2018 / Accepted: 22 December 2018 / Published: 26 December 2018
(This article belongs to the Special Issue Enteroviruses)
Enterovirus A71 (EVA71) is a human enterovirus belonging to the Picornaviridae family and mostly causes hand-foot-and-mouth disease in infants. Viperin is an important interferon-stimulated gene with a broad antiviral activity against various viruses. However, the effect of viperin on human enteroviruses and the interaction mechanism between EVA71 and viperin remains elusive. Here, we confirmed the EVA71-induced expression of viperin in a mouse model and cell lines and showed that viperin upregulation by EVA71 infection occurred on both the mRNA and protein level. Viperin knockdown and overexpression in EVA71-infected cells indicated that this protein can markedly inhibit EVA71 infection. Interestingly, immunofluorescent confocal microscopy and co-immunoprecipitation assays indicated that viperin interacts and colocalizes with the EVA71 protein 2C in the endoplasmic reticulum. Furthermore, amino acids 50–60 in the N-terminal domain of viperin were the key residues responsible for viperin interaction with 2C. More importantly, the N-terminal domain of viperin was found responsible for inhibiting EVA71 replication. Our findings can potentially aid future research on the prevention and treatment of nervous system damage caused by EVA71 and may provide a potential target for antiviral therapy. View Full-Text
Keywords: Enterovirus A71; interferon-stimulated gene; viperin; 2C protein; virus-host interaction Enterovirus A71; interferon-stimulated gene; viperin; 2C protein; virus-host interaction
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MDPI and ACS Style

Wei, C.; Zheng, C.; Sun, J.; Luo, D.; Tang, Y.; Zhang, Y.; Ke, X.; Liu, Y.; Zheng, Z.; Wang, H. Viperin Inhibits Enterovirus A71 Replication by Interacting with Viral 2C Protein. Viruses 2019, 11, 13. https://doi.org/10.3390/v11010013

AMA Style

Wei C, Zheng C, Sun J, Luo D, Tang Y, Zhang Y, Ke X, Liu Y, Zheng Z, Wang H. Viperin Inhibits Enterovirus A71 Replication by Interacting with Viral 2C Protein. Viruses. 2019; 11(1):13. https://doi.org/10.3390/v11010013

Chicago/Turabian Style

Wei, Chunyu, Caishang Zheng, Jianhong Sun, Dan Luo, Yan Tang, Yuan Zhang, Xianliang Ke, Yan Liu, Zhenhua Zheng, and Hanzhong Wang. 2019. "Viperin Inhibits Enterovirus A71 Replication by Interacting with Viral 2C Protein" Viruses 11, no. 1: 13. https://doi.org/10.3390/v11010013

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