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Open AccessArticle

Genetic and Phenotypic Characterization of Cryphonectria hypovirus 1 from Eurasian Georgia

1
WSL Swiss Federal Research Institute, Zürcherstrasse 111, 8903 Birmensdorf; Switzerland
2
Vasil Gulisashvili Forestry Institute, Agricultural University of Georgia, 0186 Tbilisi, Georgia
*
Author to whom correspondence should be addressed.
Viruses 2018, 10(12), 687; https://doi.org/10.3390/v10120687
Received: 19 October 2018 / Revised: 30 November 2018 / Accepted: 30 November 2018 / Published: 3 December 2018
(This article belongs to the Special Issue Mycoviruses)
Cryphonectria hypovirus 1 (CHV-1) infects the chestnut blight fungus Cryphonectria parasitica and acts as a biological control agent against this harmful tree disease. In this study, we screened the recently characterized C. parasitica population in Eurasian Georgia for the presence of CHV-1. We found 62 CHV-1 infected C. parasitica isolates (9.3%) among a total of 664 isolates sampled in 14 locations across Georgia. The prevalence of CHV-1 at the different locations ranged from 0% in the eastern part of the country to 29% in the western part. Sequencing of two specific regions of the viral genome one each in ORFA and ORFB revealed a unique CHV-1 subtype in Georgia. This subtype has a recombinant pattern combining the ORFA region from the subtype F2 and the ORFB region from subtype D. All 62 viral strains belonged to this Georgian CHV-1 subtype (subtype G). The CHV-1 subtype G strongly reduced the parasitic growth of C. parasitica isolates from Georgia, with a more severe effect on the European genepool compared to the Georgian genepool. The CHV-1 subtype detected in Georgia provides a valuable candidate for biological control applications in the Caucasus region. View Full-Text
Keywords: mycovirus; populations study; Cryphonectria parasitica; chestnut blight; Castanea sativa; biological control mycovirus; populations study; Cryphonectria parasitica; chestnut blight; Castanea sativa; biological control
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Rigling, D.; Borst, N.; Cornejo, C.; Supatashvili, A.; Prospero, S. Genetic and Phenotypic Characterization of Cryphonectria hypovirus 1 from Eurasian Georgia. Viruses 2018, 10, 687.

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