Next Article in Journal
A Novel Highly Divergent Strain of Cell Fusing Agent Virus (CFAV) in Mosquitoes from the Brazilian Amazon Region
Next Article in Special Issue
Genetic and Phenotypic Characterization of Cryphonectria hypovirus 1 from Eurasian Georgia
Previous Article in Journal
Virus-Derived Small Interfering RNAs Affect the Accumulations of Viral and Host Transcripts in Maize
Previous Article in Special Issue
Description, Distribution, and Relevance of Viruses of the Forest Pathogen Gremmeniella abietina
Open AccessCommunication

Mycoviral Population Dynamics in Spanish Isolates of the Entomopathogenic Fungus Beauveria bassiana

1
Department of Life Sciences, Imperial College London, London SW7 2AZ, UK
2
Department of Biological and Environmental Sciences, University of Hertfordshire, Hatfield AL10 9AB, UK
3
Department of Agricultural and Forestry Sciences, University of Cordoba, 14071 Cordoba, Spain
4
Department of Plant and Environmental Sciences, University of Copenhagen, 1871 Frederiksberg C, Denmark
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work
Viruses 2018, 10(12), 665; https://doi.org/10.3390/v10120665
Received: 26 October 2018 / Revised: 19 November 2018 / Accepted: 21 November 2018 / Published: 24 November 2018
(This article belongs to the Special Issue Mycoviruses)
The use of mycoviruses to manipulate the virulence of entomopathogenic fungi employed as biocontrol agents may lead to the development of novel methods to control attacks by insect pests. Such approaches are urgently required, as existing agrochemicals are being withdrawn from the market due to environmental and health concerns. The aim of this work is to investigate the presence and diversity of mycoviruses in large panels of entomopathogenic fungi, mostly from Spain and Denmark. In total, 151 isolates belonging to the genera Beauveria, Metarhizium, Lecanicillium, Purpureocillium, Isaria, and Paecilomyces were screened for the presence of dsRNA elements and 12 Spanish B. bassiana isolates were found to harbor mycoviruses. All identified mycoviruses belong to three previously characterised species, the officially recognised Beauveria bassiana victorivirus 1 (BbVV-1) and the proposed Beauveria bassiana partitivirus 2 (BbPV-2) and Beauveria bassiana polymycovirus 1 (BbPmV-1); individual B. bassiana isolates may harbor up to three of these mycoviruses. Notably, these mycovirus species are under distinct selection pressures, while recombination of viral genomes increases population diversity. Phylogenetic analysis of the RNA-dependent RNA polymerase gene sequences revealed that the current population structure in Spain is potentially a result of both vertical and horizontal mycovirus transmission. Finally, pathogenicity experiments using the Mediterranean fruit fly Ceratitis capitata showed no direct correlation between the presence of any particular mycovirus and the virulence of the B. bassiana isolates, but illustrated potentially interesting isolates that exhibit relatively high virulence, which will be used in more detailed virulence experimentation in the future. View Full-Text
Keywords: mycovirus; Beauveria bassiana; partitivirus; victorivirus; polymycovirus; selection pressure; recombination; transmission mycovirus; Beauveria bassiana; partitivirus; victorivirus; polymycovirus; selection pressure; recombination; transmission
Show Figures

Figure 1

MDPI and ACS Style

Filippou, C.; Garrido-Jurado, I.; Meyling, N.V.; Quesada-Moraga, E.; Coutts, R.H.A.; Kotta-Loizou, I. Mycoviral Population Dynamics in Spanish Isolates of the Entomopathogenic Fungus Beauveria bassiana. Viruses 2018, 10, 665.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop