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Sensors 2017, 17(12), 2765; https://doi.org/10.3390/s17122765

Gold Nanoparticles Used as Protein Scavengers Enhance Surface Plasmon Resonance Signal

Instituto de Ciência e Tecnologia, Universidade Federal de São Paulo, Rua Talim 330, Vila Nair, São José dos Campos, SP 12231-280, Brazil
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Received: 21 October 2017 / Revised: 20 November 2017 / Accepted: 22 November 2017 / Published: 29 November 2017
(This article belongs to the Special Issue Surface Plasmon Resonance Sensing)
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Abstract

Although several researchers had reported on methodologies for surface plasmon resonance (SPR) signal amplification based on the use of nanoparticles (NPs), the majority addressed the sandwich technique and low protein concentration. In this work, a different approach for SPR signal enhancement based on the use of gold NPs was evaluated. The method was used in the detection of two lectins, peanut agglutinin (PNA) and concanavalin A (ConA). Gold NPs were functionalized with antibodies anti-PNA and anti-ConA, and these NPs were used as protein scavengers in a solution. After being incubated with solutions of PNA or ConA, the gold NPs coupled with the collected lectins were injected on the sensor containing the immobilized antibodies. The signal amplification provided by this method was compared to the signal amplification provided by the direct coupling of PNA and ConA to gold NPs. Furthermore, both methods, direct coupling and gold NPs as protein scavengers, were compared to the direct detection of PNA and ConA in solution. Compared to the analysis of free protein, the direct coupling of PNA and ConA to gold NPs resulted in a signal amplification of 10–40-fold and a 13-fold decrease of the limit of detection (LOD), whereas the use of gold NPs as protein scavengers resulted in an SPR signal 40–50-times higher and an LOD 64-times lower. View Full-Text
Keywords: surface plasmon resonance; gold nanoparticles; signal amplification; lectins; concanavalin A; peanut agglutinin surface plasmon resonance; gold nanoparticles; signal amplification; lectins; concanavalin A; peanut agglutinin
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Ferreira de Macedo, E.; Ducatti Formaggio, D.M.; Salles Santos, N.; Batista Tada, D. Gold Nanoparticles Used as Protein Scavengers Enhance Surface Plasmon Resonance Signal. Sensors 2017, 17, 2765.

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