New Formulation of Platelet-Rich Plasma Enriched in Platelet and Extraplatelet Biomolecules Using Hydrogels
Abstract
:1. Introduction
2. Results
2.1. Water Absorption Parameters Optimization for nPRP Obtention
2.2. Comparison of Platelets and Total Proteins Concentration Capacity between a Standard (sPRP) and a Novel (nPRP) PRP
2.3. Concentration of Growth Factors in sPRP and nPRP
2.4. Ion Concentration Capacity of sPRP and nPRP
2.5. sPRP and nPRP Processing Impact on Platelet Activation
2.6. Fibrinogen Levels and Biomechanical Properties of the Clot Resulting from sPRP and nPRP
2.7. Viability of Fibroblasts Culture upon Exposure to nPRP
3. Discussion
4. Materials and Methods
4.1. Donors
4.2. Fabrication of the Hydroxyethyl Acrylamide (HEAA)-Based Hydrogel Powder
4.3. Standard Platelet-Rich Plasma (sPRP) Preparation
4.4. Novel Platelet-Rich Plasma (nPRP) Preparation
4.5. Platelet and Protein Fold Measurement
4.6. Platelet and Plasmatic Growth Factor Concentration Measurement
4.7. Ion Levels Measurement
4.8. Platelet Activation Test
4.9. Fibrinogen Levels Measurements and Biomechanical Tests on Fibrin Clots
4.10. Normal Human Dermal Fibroblast (NHDF) Cell Cultures
4.11. Isolation and Culture of Human Primary Chondrocytes
4.12. Cell Viability Assay
4.13. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Mercader Ruiz, J.; Beitia, M.; Delgado, D.; Sánchez, P.; Arnaiz, M.J.; López de Dicastillo, L.; Benito-Lopez, F.; Basabe-Desmonts, L.; Sánchez, M. New Formulation of Platelet-Rich Plasma Enriched in Platelet and Extraplatelet Biomolecules Using Hydrogels. Int. J. Mol. Sci. 2023, 24, 13811. https://doi.org/10.3390/ijms241813811
Mercader Ruiz J, Beitia M, Delgado D, Sánchez P, Arnaiz MJ, López de Dicastillo L, Benito-Lopez F, Basabe-Desmonts L, Sánchez M. New Formulation of Platelet-Rich Plasma Enriched in Platelet and Extraplatelet Biomolecules Using Hydrogels. International Journal of Molecular Sciences. 2023; 24(18):13811. https://doi.org/10.3390/ijms241813811
Chicago/Turabian StyleMercader Ruiz, Jon, Maider Beitia, Diego Delgado, Pello Sánchez, María Jesús Arnaiz, Leonor López de Dicastillo, Fernando Benito-Lopez, Lourdes Basabe-Desmonts, and Mikel Sánchez. 2023. "New Formulation of Platelet-Rich Plasma Enriched in Platelet and Extraplatelet Biomolecules Using Hydrogels" International Journal of Molecular Sciences 24, no. 18: 13811. https://doi.org/10.3390/ijms241813811