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Volume 22
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Article

Comparison of Decellularization Protocols to Generate Peripheral Nerve Grafts: A Study on Rat Sciatic Nerves

by
Marwa El Soury
1,2,3,†,
Óscar Darío García-García
3,4,†,
Matteo Moretti
5,6,
Isabelle Perroteau
1,
Stefania Raimondo
1,2,*,
Arianna Barbara Lovati
5,‡ and
Víctor Carriel
3,4,‡
1
Department of Clinical and Biological Sciences, University of Torino, 10043 Orbassano, Italy
2
Neuroscience Institute Cavalieri Ottolenghi (NICO), University of Torino, 10043 Orbassano, Italy
3
Tissue Engineering Group, Department of Histology, University of Granada, 18012 Granada, Spain
4
Instituto de Investigacion Biosanitaria, Ibs.GRANADA, 18012 Granada, Spain
5
IRCCS Istituto Ortopedico Galeazzi, Cell and Tissue Engineering Laboratory, 20161 Milan, Italy
6
Regenerative Medicine Technologies Laboratory, Ente Ospedaliero Cantonale, 6900 Lugano, Switzerland
*
Author to whom correspondence should be addressed.
†
These authors have contributed equally to this work and share first authorship.
‡
These authors share senior authorship.
Int. J. Mol. Sci. 2021, 22(5), 2389; https://doi.org/10.3390/ijms22052389
Submission received: 29 January 2021 / Revised: 23 February 2021 / Accepted: 24 February 2021 / Published: 27 February 2021
(This article belongs to the Special Issue Regenerative Medicine for Peripheral Nerve Injury: Recent Advances, Emerging Therapies and Future Directions)
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Abstract

In critical nerve gap repair, decellularized nerve allografts are considered a promising tissue engineering strategy that can provide superior regeneration results compared to nerve conduits. Decellularized nerves offer a well-conserved extracellular matrix component that has proven to play an important role in supporting axonal guiding and peripheral nerve regeneration. Up to now, the known decellularized techniques are time and effort consuming. The present study, performed on rat sciatic nerves, aims at investigating a novel nerve decellularization protocol able to combine an effective decellularization in short time with a good preservation of the extracellular matrix component. To do this, a decellularization protocol proven to be efficient for tendons (DN-P1) was compared with a decellularization protocol specifically developed for nerves (DN-P2). The outcomes of both the decellularization protocols were assessed by a series of in vitro evaluations, including qualitative and quantitative histological and immunohistochemical analyses, DNA quantification, SEM and TEM ultrastructural analyses, mechanical testing, and viability assay. The overall results showed that DN-P1 could provide promising results if tested in vivo, as the in vitro characterization demonstrated that DN-P1 conserved a better ultrastructure and ECM components compared to DN-P2. Most importantly, DN-P1 was shown to be highly biocompatible, supporting a greater number of viable metabolically active cells.
Keywords: peripheral nerves; decellularization; acellular; regeneration; orthopedic trauma; extracellular matrix peripheral nerves; decellularization; acellular; regeneration; orthopedic trauma; extracellular matrix

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MDPI and ACS Style

El Soury, M.; García-García, Ó.D.; Moretti, M.; Perroteau, I.; Raimondo, S.; Lovati, A.B.; Carriel, V. Comparison of Decellularization Protocols to Generate Peripheral Nerve Grafts: A Study on Rat Sciatic Nerves. Int. J. Mol. Sci. 2021, 22, 2389. https://doi.org/10.3390/ijms22052389

AMA Style

El Soury M, García-García ÓD, Moretti M, Perroteau I, Raimondo S, Lovati AB, Carriel V. Comparison of Decellularization Protocols to Generate Peripheral Nerve Grafts: A Study on Rat Sciatic Nerves. International Journal of Molecular Sciences. 2021; 22(5):2389. https://doi.org/10.3390/ijms22052389

Chicago/Turabian Style

El Soury, Marwa, Óscar Darío García-García, Matteo Moretti, Isabelle Perroteau, Stefania Raimondo, Arianna Barbara Lovati, and Víctor Carriel. 2021. "Comparison of Decellularization Protocols to Generate Peripheral Nerve Grafts: A Study on Rat Sciatic Nerves" International Journal of Molecular Sciences 22, no. 5: 2389. https://doi.org/10.3390/ijms22052389

APA Style

El Soury, M., García-García, Ó. D., Moretti, M., Perroteau, I., Raimondo, S., Lovati, A. B., & Carriel, V. (2021). Comparison of Decellularization Protocols to Generate Peripheral Nerve Grafts: A Study on Rat Sciatic Nerves. International Journal of Molecular Sciences, 22(5), 2389. https://doi.org/10.3390/ijms22052389

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