Next Article in Journal
Thrombin-Binding Aptamer with Inversion of Polarity Sites (IPS): Effect on DNAzyme Activity and Anticoagulant Properties
Next Article in Special Issue
Injury-Induced HSP27 Expression in Peripheral Nervous Tissue Is Not Associated with Any Alteration in Axonal Outgrowth after Immediate or Delayed Nerve Repair
Previous Article in Journal
Tying Small Changes to Large Outcomes: The Cautious Promise in Incorporating the Microbiome into Immunotherapy
Previous Article in Special Issue
Toll-Like Receptor 9-Mediated Neuronal Innate Immune Reaction Is Associated with Initiating a Pro-Regenerative State in Neurons of the Dorsal Root Ganglia Non-Associated with Sciatic Nerve Lesion
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 22
Issue 15
10.3390/ijms22157901
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Chitosan Micro-Grooved Membranes with Increased Asymmetry for the Improvement of the Schwann Cell Response in Nerve Regeneration

by
Luca Scaccini
1,†,
Roberta Mezzena
1,†,
Alessia De Masi
1,
Mariacristina Gagliardi
1,
Giovanna Gambarotta
2,3,
Marco Cecchini
1 and
Ilaria Tonazzini
1,*
1
National Enterprise for Nanoscience and Nanotechnology (NEST), Istituto Nanoscienze Consiglio Nazionale delle Ricerche (CNR) and Scuola Normale Superiore Pisa, Piazza San Silvestro 12, 56127 Pisa, Italy
2
Department of Clinical and Biological Sciences (DSCB), University of Torino, Orbassano, 10043 Turin, Italy
3
Neuroscience Institute Cavalieri Ottolenghi (NICO), Regione Gonzole 10, Orbassano, 10043 Turin, Italy
*
Author to whom correspondence should be addressed.
†
These authors equally contributed to the work.
Int. J. Mol. Sci. 2021, 22(15), 7901; https://doi.org/10.3390/ijms22157901
Submission received: 10 June 2021 / Revised: 14 July 2021 / Accepted: 19 July 2021 / Published: 23 July 2021
(This article belongs to the Special Issue Regenerative Medicine for Peripheral Nerve Injury: Recent Advances, Emerging Therapies and Future Directions)
Browse Figures
Versions Notes

Abstract

Peripheral nerve injuries are a common condition in which a nerve is damaged, affecting more than one million people every year. There are still no efficient therapeutic treatments for these injuries. Artificial scaffolds can offer new opportunities for nerve regeneration applications; in this framework, chitosan is emerging as a promising biomaterial. Here, we set up a simple and effective method for the production of micro-structured chitosan films by solvent casting, with high fidelity in the micro-pattern reproducibility. Three types of chitosan directional micro-grooved patterns, presenting different levels of symmetricity, were developed for application in nerve regenerative medicine: gratings (GR), isosceles triangles (ISO) and scalene triangles (SCA). The directional patterns were tested with a Schwann cell line. The most asymmetric topography (SCA), although it polarized the cell shaping less efficiently, promoted higher cell proliferation and a faster cell migration, both individually and collectively, with a higher directional persistence of motion. Overall, the use of micro-structured asymmetrical directional topographies may be exploited to enhance the nerve regeneration process mediated by chitosan scaffolds.
Keywords: contact guidance; Schwann cells; nerve regeneration contact guidance; Schwann cells; nerve regeneration

Share and Cite

MDPI and ACS Style

Scaccini, L.; Mezzena, R.; De Masi, A.; Gagliardi, M.; Gambarotta, G.; Cecchini, M.; Tonazzini, I. Chitosan Micro-Grooved Membranes with Increased Asymmetry for the Improvement of the Schwann Cell Response in Nerve Regeneration. Int. J. Mol. Sci. 2021, 22, 7901. https://doi.org/10.3390/ijms22157901

AMA Style

Scaccini L, Mezzena R, De Masi A, Gagliardi M, Gambarotta G, Cecchini M, Tonazzini I. Chitosan Micro-Grooved Membranes with Increased Asymmetry for the Improvement of the Schwann Cell Response in Nerve Regeneration. International Journal of Molecular Sciences. 2021; 22(15):7901. https://doi.org/10.3390/ijms22157901

Chicago/Turabian Style

Scaccini, Luca, Roberta Mezzena, Alessia De Masi, Mariacristina Gagliardi, Giovanna Gambarotta, Marco Cecchini, and Ilaria Tonazzini. 2021. "Chitosan Micro-Grooved Membranes with Increased Asymmetry for the Improvement of the Schwann Cell Response in Nerve Regeneration" International Journal of Molecular Sciences 22, no. 15: 7901. https://doi.org/10.3390/ijms22157901

APA Style

Scaccini, L., Mezzena, R., De Masi, A., Gagliardi, M., Gambarotta, G., Cecchini, M., & Tonazzini, I. (2021). Chitosan Micro-Grooved Membranes with Increased Asymmetry for the Improvement of the Schwann Cell Response in Nerve Regeneration. International Journal of Molecular Sciences, 22(15), 7901. https://doi.org/10.3390/ijms22157901

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop