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In Silico and In Vitro Analyses Validate Human MicroRNAs Targeting the SARS-CoV-2 3′-UTR

Molecular Neuroprotection Group, Research Unit, National Hospital for Paraplegics (SESCAM), 45071 Toledo, Spain
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Academic Editor: James K. Bashkin
Int. J. Mol. Sci. 2021, 22(11), 6094; https://doi.org/10.3390/ijms22116094
Received: 25 March 2021 / Revised: 24 May 2021 / Accepted: 29 May 2021 / Published: 5 June 2021
(This article belongs to the Section Molecular Biology)
COVID-19 pandemic is caused by betacoronavirus SARS-CoV-2. The genome of this virus is composed of a single strand of RNA with 5′ and 3′-UTR flanking a region of protein-coding ORFs closely resembling cells’ mRNAs. MicroRNAs are endogenous post-transcriptional regulators that target mRNA to modulate protein expression and mediate cellular functions, including antiviral defense. In the present study, we carried out a bioinformatics screening to search for endogenous human microRNAs targeting the 3′-UTR of SARS-CoV-2. Results from the computational techniques allowed us to identify 10 potential candidates. The capacity of 3 of them, together with hsa-miR-138-5p, to target the SARS-CoV-2 3′-UTR was validated in vitro by gene reporter assays. Available information indicates that two of these microRNAs, namely, hsa-miR-3941 and hsa-miR-138-5p, combine effective targeting of SARS-CoV-2 genome with complementary antiviral or protective effects in the host cells that make them potential candidates for therapeutic treatment of most, if not all, COVID-19 variants known to date. All information obtained while conducting the present analysis is available at Open Science Framework repository. View Full-Text
Keywords: SARS-CoV-2 3′-UTR; hsa-miR-3941; hsa-miR-138-5p; antiviral defense SARS-CoV-2 3′-UTR; hsa-miR-3941; hsa-miR-138-5p; antiviral defense
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MDPI and ACS Style

Barreda-Manso, M.A.; Nieto-Díaz, M.; Soto, A.; Muñoz-Galdeano, T.; Reigada, D.; Maza, R.M. In Silico and In Vitro Analyses Validate Human MicroRNAs Targeting the SARS-CoV-2 3′-UTR. Int. J. Mol. Sci. 2021, 22, 6094. https://doi.org/10.3390/ijms22116094

AMA Style

Barreda-Manso MA, Nieto-Díaz M, Soto A, Muñoz-Galdeano T, Reigada D, Maza RM. In Silico and In Vitro Analyses Validate Human MicroRNAs Targeting the SARS-CoV-2 3′-UTR. International Journal of Molecular Sciences. 2021; 22(11):6094. https://doi.org/10.3390/ijms22116094

Chicago/Turabian Style

Barreda-Manso, María Asunción, Manuel Nieto-Díaz, Altea Soto, Teresa Muñoz-Galdeano, David Reigada, and Rodrigo M. Maza. 2021. "In Silico and In Vitro Analyses Validate Human MicroRNAs Targeting the SARS-CoV-2 3′-UTR" International Journal of Molecular Sciences 22, no. 11: 6094. https://doi.org/10.3390/ijms22116094

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