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RT-qPCR Testing of SARS-CoV-2: A Primer

Medical Technology Research Centre, Faculty of Health, Education, Medicine and Social Care, Anglia Ruskin University, Chelmsford, Essex CM1 1SQ, UK
Institute of Population Health, Faculty of Medical and Human Sciences, University of Manchester; Manchester M13 9NT, UK
The Gene Team, Bury St Edmunds, Suffolk IP31 1AA, UK
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(8), 3004;
Received: 13 April 2020 / Revised: 22 April 2020 / Accepted: 23 April 2020 / Published: 24 April 2020
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
Testing for the presence of coronavirus is an essential diagnostic tool for monitoring and managing the current COVID-19 pandemic. The only reliable test in current use for testing acute infection targets the genome of SARS-CoV-2, and the most widely used method is quantitative fluorescence-based reverse transcription polymerase chain reaction (RT-qPCR). Despite its ubiquity, there is a significant amount of uncertainty about how this test works, potential throughput and reliability. This has resulted in widespread misrepresentation of the problems faced using this test during the current COVID-19 epidemic. This primer provides simple, straightforward and impartial information about RT-qPCR. View Full-Text
Keywords: COVID-19; SARS; pandemic; reverse transcription; real-time fluorescence PCR COVID-19; SARS; pandemic; reverse transcription; real-time fluorescence PCR
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MDPI and ACS Style

Bustin, S.A.; Nolan, T. RT-qPCR Testing of SARS-CoV-2: A Primer. Int. J. Mol. Sci. 2020, 21, 3004.

AMA Style

Bustin SA, Nolan T. RT-qPCR Testing of SARS-CoV-2: A Primer. International Journal of Molecular Sciences. 2020; 21(8):3004.

Chicago/Turabian Style

Bustin, Stephen A., and Tania Nolan. 2020. "RT-qPCR Testing of SARS-CoV-2: A Primer" International Journal of Molecular Sciences 21, no. 8: 3004.

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