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Open AccessArticle

Sustained Release of Decoy Wnt Receptor (sLRP6E1E2)-Expressing Adenovirus Using Gel-Encapsulation for Scar Remodeling in Pig Model

1
Department of Plastic & Reconstructive Surgery, Wonju College of Medicine, Yonsei University, Wonju 26426, Korea
2
Institute for Human Tissue Restoration, Department of Plastic & Reconstructive Surgery, Yonsei University College of Medicine, Seoul 03722, Korea
3
Department of Dermatology and Cutaneous Biology Research Institute, Yonsei University College of Medicine, Seoul 03722, Korea
4
Department of Bioengineering, College of Engineering, Hanyang University, Seoul 04763, Korea
5
Institute of Nano Science and Technology (INST), Hanyang University, Seoul 04763, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(6), 2242; https://doi.org/10.3390/ijms21062242
Received: 10 February 2020 / Revised: 10 March 2020 / Accepted: 20 March 2020 / Published: 24 March 2020
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
An adenoviral vector (Ad) expressing a Wnt decoy receptor (sLRP6E1E2) is known to induce an anti-fibrotic effect by inhibiting Wnt signaling. We evaluated its effects in vivo using pig models and attempted to introduce an alginate gel-matrix system to prolong the effect of the Ad. Transduction efficiency as to the biological activity of Ad in different forms was evaluated. Then, 50 days after the formation of full-thickness skin defects on the backs of Yorkshire pigs, scars were treated with each form of Ad. Therapeutic efficacy and various factors influencing scar formation and collagen rearrangement were analyzed. Inflammatory cell infiltration within the scar tissues was also evaluated. Decoy Wnt receptor (sLRP6E1E2)-expressing adenovirus treatment improved scar quality in a pig model. Loading this construct in alginate gel allows sustained virus release into local tissues and prolongs Ad activity, thus maintaining its therapeutic effect longer in vivo. View Full-Text
Keywords: gene therapy; adenovirus; scar; alginate gel; decoy Wnt receptor; pig model gene therapy; adenovirus; scar; alginate gel; decoy Wnt receptor; pig model
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Yang, C.-E.; Choi, S.; Lee, J.H.; Kang, E.H.; Ahn, H.M.; Roh, T.S.; Yun, C.-O.; Lee, W.J. Sustained Release of Decoy Wnt Receptor (sLRP6E1E2)-Expressing Adenovirus Using Gel-Encapsulation for Scar Remodeling in Pig Model. Int. J. Mol. Sci. 2020, 21, 2242.

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