Overexpression of Grapevine VvIAA18 Gene Enhanced Salt Tolerance in Tobacco
Abstract
:1. Introduction
2. Results
2.1. Cloning and Sequence Analysis of VvIAA18
2.2. Nuclear Localization of VvIAA18
2.3. Transcriptional Activation of VvIAA18
2.4. Improved Salt Tolerance in Escherichia coli
2.5. Regeneration and Identification of Transgenic Tobacco with VvIAA18 Gene
2.6. Enhanced Salt Tolerance in Tobacco Expressing VvIAA18
2.7. Southern Blot Analysis of Transgenic Plants
2.8. Increased Expression of the Salt Stress-Responsive Genes in Transgenic Plants
2.9. Promoted Stress Response Physiological Traits in Transgenic Plants
3. Discussion
4. Materials and Methods
4.1. Plant Mterials
4.2. Cloning of Grapevine VvIAA18 Gene
4.3. Sequence Analysis of VvIAA18 Gene
4.4. Subcellular Localization and Transactivation Assay of VvIAA18
4.5. Protein Expression and Bacterial Growth Analysis
4.6. Transformation of Tobacco with VvIAA18
4.7. Molecular Confirmation of Transgenic Plants
4.8. Assay for Salt Tolerance
4.9. Southern Blot Analysis
4.10. Expression Analysis of VvIAA18 and the Related Genes
4.11. Analyses of Proline, H2O2, and MDA Content and SOD and POD Activities
4.12. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Li, W.; Dang, C.; Ye, Y.; Wang, Z.; Hu, L.; Zhang, F.; Zhang, Y.; Qian, X.; Shi, J.; Guo, Y.; et al. Overexpression of Grapevine VvIAA18 Gene Enhanced Salt Tolerance in Tobacco. Int. J. Mol. Sci. 2020, 21, 1323. https://doi.org/10.3390/ijms21041323
Li W, Dang C, Ye Y, Wang Z, Hu L, Zhang F, Zhang Y, Qian X, Shi J, Guo Y, et al. Overexpression of Grapevine VvIAA18 Gene Enhanced Salt Tolerance in Tobacco. International Journal of Molecular Sciences. 2020; 21(4):1323. https://doi.org/10.3390/ijms21041323
Chicago/Turabian StyleLi, Wei, Changxi Dang, Yuxiu Ye, Zunxin Wang, Laibao Hu, Fan Zhang, Yang Zhang, Xingzhi Qian, Jiabin Shi, Yanyun Guo, and et al. 2020. "Overexpression of Grapevine VvIAA18 Gene Enhanced Salt Tolerance in Tobacco" International Journal of Molecular Sciences 21, no. 4: 1323. https://doi.org/10.3390/ijms21041323