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Open AccessArticle

Hepatitis C Virus p7 Induces Membrane Permeabilization by Interacting with Phosphatidylserine

1
Laboratory of Molecular Cell Biology, Graduate School of Medicine, Korea University College of Medicine, Korea University, Seoul 02841, Korea
2
Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Korea
3
Department of Surgery, Seoul National University College of Medicine, Seoul 03080, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(3), 897; https://doi.org/10.3390/ijms21030897
Received: 2 January 2020 / Revised: 26 January 2020 / Accepted: 28 January 2020 / Published: 30 January 2020
(This article belongs to the Special Issue Lipid-Protein and Protein-Protein Interactions in Membranes)
Hepatitis C virus (HCV) p7 is known to be a nonselective cation channel for HCV maturation. Because the interaction of HCV proteins with host lipids in the endoplasmic reticulum membrane is crucial for the budding process, the identification of p7–lipid interactions could be important for understanding the HCV life cycle. Here, we report that p7 interacts with phosphatidylserine (PS) to induce membrane permeabilization. The interaction of p7 with PS was not inhibited by Gd3+ ions, which have been known to interact with negatively charged lipids, but channel activity and p7-induced mitochondrial depolarization were inhibited by Gd3+ ions. From the present results, we suggest that the p7–PS interaction plays an essential role in regulating its ion channel function and could be a potential molecular target for anti-HCV therapy. View Full-Text
Keywords: Hepatitis C virus; p7; membrane permeabilization; phosphatidylserine; gadolinium Hepatitis C virus; p7; membrane permeabilization; phosphatidylserine; gadolinium
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Lee, H.-R.; Lee, G.Y.; You, D.-G.; Kim, H.K.; Yoo, Y.D. Hepatitis C Virus p7 Induces Membrane Permeabilization by Interacting with Phosphatidylserine. Int. J. Mol. Sci. 2020, 21, 897.

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