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Open AccessReview

Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for Research and Biomedical Applications

1
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 660036 Krasnoyarsk, Russia
2
School of Fundamental Biology and Biotechnology, Siberian Federal University, 660041 Krasnoyarsk, Russia
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(20), 7465; https://doi.org/10.3390/ijms21207465
Received: 28 August 2020 / Revised: 5 October 2020 / Accepted: 7 October 2020 / Published: 10 October 2020
The functioning of bioluminescent systems in most of the known marine organisms is based on the oxidation reaction of the same substrate—coelenterazine (CTZ), catalyzed by luciferase. Despite the diversity in structures and the functioning mechanisms, these enzymes can be united into a common group called CTZ-dependent luciferases. Among these, there are two sharply different types of the system organization—Ca2+-regulated photoproteins and luciferases themselves that function in accordance with the classical enzyme–substrate kinetics. Along with deep and comprehensive fundamental research on these systems, approaches and methods of their practical use as highly sensitive reporters in analytics have been developed. The research aiming at the creation of artificial luciferases and synthetic CTZ analogues with new unique properties has led to the development of new experimental analytical methods based on them. The commercial availability of many ready-to-use assay systems based on CTZ-dependent luciferases is also important when choosing them by first-time-users. The development of analytical methods based on these bioluminescent systems is currently booming. The bioluminescent systems under consideration were successfully applied in various biological research areas, which confirms them to be a powerful analytical tool. In this review, we consider the main directions, results, and achievements in research involving these luciferases. View Full-Text
Keywords: bioluminescence; coelenterazine; luciferase; Ca2+-regulated photoprotein; analytical systems bioluminescence; coelenterazine; luciferase; Ca2+-regulated photoprotein; analytical systems
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MDPI and ACS Style

Krasitskaya, V.V.; Bashmakova, E.E.; Frank, L.A. Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for Research and Biomedical Applications. Int. J. Mol. Sci. 2020, 21, 7465. https://doi.org/10.3390/ijms21207465

AMA Style

Krasitskaya VV, Bashmakova EE, Frank LA. Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for Research and Biomedical Applications. International Journal of Molecular Sciences. 2020; 21(20):7465. https://doi.org/10.3390/ijms21207465

Chicago/Turabian Style

Krasitskaya, Vasilisa V.; Bashmakova, Eugenia E.; Frank, Ludmila A. 2020. "Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for Research and Biomedical Applications" Int. J. Mol. Sci. 21, no. 20: 7465. https://doi.org/10.3390/ijms21207465

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