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Open AccessArticle

Influence of the Casting Concentration on the Mechanical and Optical Properties of FA/CaCl2-Derived Silk Fibroin Membranes

1
Fibrothelium GmbH, 52068 Aachen, Germany
2
Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg Eppendorf, 20251 Hamburg, Germany
3
Department of Oral and Maxillofacial Surgery, Division of Regenerative Orofacial Medicine, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany
4
Department of Plastic, Reconstructive and Aesthetic Surgery, University Hospital of Cologne, 52074 Cologne, Germany
5
Polyclinic for Dental Prosthetics, University Medical Center Göttingen, 37075 Göttingen, Germany
6
University of Applied Sciences, FH Aachen, 52428 Jülich, Germany
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(18), 6704; https://doi.org/10.3390/ijms21186704
Received: 11 August 2020 / Revised: 7 September 2020 / Accepted: 10 September 2020 / Published: 13 September 2020
In this study, we describe the manufacturing and characterization of silk fibroin membranes derived from the silkworm Bombyx mori. To date, the dissolution process used in this study has only been researched to a limited extent, although it entails various potential advantages, such as reduced expenses and the absence of toxic chemicals in comparison to other conventional techniques. Therefore, the aim of this study was to determine the influence of different fibroin concentrations on the process output and resulting membrane properties. Casted membranes were thus characterized with regard to their mechanical, structural and optical assets via tensile testing, SEM, light microscopy and spectrophotometry. Cytotoxicity was evaluated using BrdU, XTT, and LDH assays, followed by live–dead staining. The formic acid (FA) dissolution method was proven to be suitable for the manufacturing of transparent and mechanically stable membranes. The fibroin concentration affects both thickness and transparency of the membranes. The membranes did not exhibit any signs of cytotoxicity. When compared to other current scientific and technical benchmarks, the manufactured membranes displayed promising potential for various biomedical applications. Further research is nevertheless necessary to improve reproducible manufacturing, including a more uniform thickness, less impurity and physiological pH within the membranes. View Full-Text
Keywords: silk; membrane; fibroin; biopolymer; tissue regeneration silk; membrane; fibroin; biopolymer; tissue regeneration
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MDPI and ACS Style

Kopp, A.; Schunck, L.; Gosau, M.; Smeets, R.; Burg, S.; Fuest, S.; Kröger, N.; Zinser, M.; Krohn, S.; Behbahani, M.; Köpf, M.; Lauts, L.; Rutkowski, R. Influence of the Casting Concentration on the Mechanical and Optical Properties of FA/CaCl2-Derived Silk Fibroin Membranes. Int. J. Mol. Sci. 2020, 21, 6704. https://doi.org/10.3390/ijms21186704

AMA Style

Kopp A, Schunck L, Gosau M, Smeets R, Burg S, Fuest S, Kröger N, Zinser M, Krohn S, Behbahani M, Köpf M, Lauts L, Rutkowski R. Influence of the Casting Concentration on the Mechanical and Optical Properties of FA/CaCl2-Derived Silk Fibroin Membranes. International Journal of Molecular Sciences. 2020; 21(18):6704. https://doi.org/10.3390/ijms21186704

Chicago/Turabian Style

Kopp, Alexander; Schunck, Laura; Gosau, Martin; Smeets, Ralf; Burg, Simon; Fuest, Sandra; Kröger, Nadja; Zinser, Max; Krohn, Sebastian; Behbahani, Mehdi; Köpf, Marius; Lauts, Lisa; Rutkowski, Rico. 2020. "Influence of the Casting Concentration on the Mechanical and Optical Properties of FA/CaCl2-Derived Silk Fibroin Membranes" Int. J. Mol. Sci. 21, no. 18: 6704. https://doi.org/10.3390/ijms21186704

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