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Article

Production of Injectable Marine Collagen-Based Hydrogel for the Maintenance of Differentiated Chondrocytes in Tissue Engineering Applications

1
Abiel s.r.l, c/o University of Palermo, Viale delle Scienze, Ed. 16, 90128 Palermo, Italy
2
Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Viale delle Scienze, Ed. 16, 90128 Palermo, Italy
3
Institute for Biomedical Research and Innovation-National Research Council (IRIB-CNR), Via Ugo La Malfa 153, 90146 Palermo, Italy
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(16), 5798; https://doi.org/10.3390/ijms21165798
Received: 13 July 2020 / Revised: 7 August 2020 / Accepted: 10 August 2020 / Published: 12 August 2020
Cartilage is an avascular tissue with limited ability of self-repair. The use of autologous chondrocyte transplants represent an effective strategy for cell regeneration; however, preserving the differentiated state, which ensures the ability to regenerate damaged cartilage, represents the main challenge during in vitro culturing. For this purpose, we produced an injectable marine collagen-based hydrogel, by mixing native collagen from the jellyfish Rhizostoma pulmo with hydroxy-phenyl-propionic acid (HPA)-functionalized marine gelatin. This biocompatible hydrogel formulation, due to the ability of enzymatically reticulate using horseradish peroxidase (HPR) and H2O2, gives the possibility of trap cells inside, in the absence of cytotoxic effects, during the cross-linking process. Moreover, it enables the modulation of the hydrogel stiffness merely varying the concentration of H2O2 without changes in the concentration of polymer precursors. The maintenance of differentiated chondrocytes in culture was then evaluated via morphological analysis of cell phenotype, GAG production and cytoskeleton organization. Additionally, gene expression profiling of differentiation/dedifferentiation markers provided evidence for the promotion of the chondrogenic gene expression program. This, combined with the biochemical properties of marine collagen, represents a promising strategy for maintaining in vitro the cellular phenotype in the aim of the use of autologous chondrocytes in regenerative medicine practices. View Full-Text
Keywords: jellyfish collagen; composite injectable hydrogel; enzymatic cross-linking; cartilage; chondrocytes; differentiation; gene expression jellyfish collagen; composite injectable hydrogel; enzymatic cross-linking; cartilage; chondrocytes; differentiation; gene expression
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MDPI and ACS Style

Rigogliuso, S.; Salamone, M.; Barbarino, E.; Barbarino, M.; Nicosia, A.; Ghersi, G. Production of Injectable Marine Collagen-Based Hydrogel for the Maintenance of Differentiated Chondrocytes in Tissue Engineering Applications. Int. J. Mol. Sci. 2020, 21, 5798. https://doi.org/10.3390/ijms21165798

AMA Style

Rigogliuso S, Salamone M, Barbarino E, Barbarino M, Nicosia A, Ghersi G. Production of Injectable Marine Collagen-Based Hydrogel for the Maintenance of Differentiated Chondrocytes in Tissue Engineering Applications. International Journal of Molecular Sciences. 2020; 21(16):5798. https://doi.org/10.3390/ijms21165798

Chicago/Turabian Style

Rigogliuso, Salvatrice, Monica Salamone, Enza Barbarino, Maria Barbarino, Aldo Nicosia, and Giulio Ghersi. 2020. "Production of Injectable Marine Collagen-Based Hydrogel for the Maintenance of Differentiated Chondrocytes in Tissue Engineering Applications" International Journal of Molecular Sciences 21, no. 16: 5798. https://doi.org/10.3390/ijms21165798

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