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Open AccessArticle

Phenolic Compounds with Antioxidant Properties from Canola Meal Extracts Inhibit Adipogenesis

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ARC Industrial Transformation Training Centre for Functional Grains & Graham Centre for Agricultural Innovation, Boorooma Street, Wagga Wagga, NSW 2650, Australia
2
Graham Centre (an alliance between Charles Sturt University and NSW Department of Primary Industries), Boorooma Street, Wagga Wagga, NSW 2678, Australia
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School of Biomedical Sciences, Charles Sturt University, Boorooma Street, Wagga Wagga, NSW 2678, Australia
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School of Medical and Applied Sciences, Central Queensland University, Rockhampton, Qld 4702, Australia
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(1), 1; https://doi.org/10.3390/ijms21010001
Received: 20 November 2019 / Revised: 5 December 2019 / Accepted: 11 December 2019 / Published: 18 December 2019
(This article belongs to the Section Bioactives and Nutraceuticals)
The extraction of phenolic compounds from canola meal produces functional health products and renders the canola meal a more digestible animal feed. The extracted phenolics may have novel bioactivity worth investigation. In this study, several solvents were evaluated for their ability to extract phenolic compounds from canola meal: water (WE) and various 80% organic solvent/water mixtures of methanol (ME), acetone (AE), ethanol (EE), butanol (BE), chloroform (CE) and hexane (HE). The in vitro antioxidant and anti-obesity properties of various extracts were investigated. Anti-obesity properties were studied using adipogenic differentiation inhibition of a murine mesenchymal stem cell line (C3H10T1/2) and a pancreatic lipase inhibition assay. AE, ME, and BE showed significant (p < 0.05) adipogenesis and pancreatic lipase inhibitory activities and may have more pharmacological properties. AE down-regulated the gene expression of the major adipogenic transcription factor, peroxisome proliferator-activated receptor gamma (PPARγ), correlating to phenolic content in a dose-dependent manner. The chemical characterization of AE revealed the presence of sinapic acid, ferulic acid, and kaempferol derivatives as main bioactive phenols. View Full-Text
Keywords: Brassica napus; rapeseed; polyphenols; adipogenesis; pancreatic lipase; antioxidant Brassica napus; rapeseed; polyphenols; adipogenesis; pancreatic lipase; antioxidant
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MDPI and ACS Style

Hussain, S.; Rehman, A.U.; Luckett, D.J.; Blanchard, C.L.; Obied, H.K.; Strappe, P. Phenolic Compounds with Antioxidant Properties from Canola Meal Extracts Inhibit Adipogenesis. Int. J. Mol. Sci. 2020, 21, 1.

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