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Int. J. Mol. Sci. 2018, 19(9), 2679; https://doi.org/10.3390/ijms19092679

Multiple Aspects of PIP2 Involvement in C. elegans Gametogenesis

1
Department of Biology of the Cell Nucleus, Institute of Molecular Genetics of the Czech Academy of Sciences, v.v.i., Prague 142 20, Czech Republic
2
Department of Epigenetics of the Cell Nucleus, Institute of Molecular Genetics of the Czech Academy of Sciences, v.v.i., Division BIOCEV, Vestec 252 50, Czech Republic
3
Microscopy Centre, Institute of Molecular Genetics of the Czech Academy of Sciences, v.v.i., Prague 142 20, Czech Republic
*
Author to whom correspondence should be addressed.
Received: 1 August 2018 / Revised: 28 August 2018 / Accepted: 5 September 2018 / Published: 10 September 2018
(This article belongs to the Special Issue Nuclear Lipid Signaling)
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Abstract

One of the most studied phosphoinositides is phosphatidylinositol 4,5-bisphosphate (PIP2), which localizes to the plasma membrane, nuclear speckles, small foci in the nucleoplasm, and to the nucleolus in mammalian cells. Here, we show that PIP2 also localizes to the nucleus in prophase I, during the gametogenesis of C. elegans hermaphrodite. The depletion of PIP2 by type I PIP kinase (PPK-1) kinase RNA interference results in an altered chromosome structure and leads to various defects during meiotic progression. We observed a decreased brood size and aneuploidy in progeny, defects in synapsis, and crossover formation. The altered chromosome structure is reflected in the increased transcription activity of a tightly regulated process in prophase I. To elucidate the involvement of PIP2 in the processes during the C. elegans development, we identified the PIP2-binding partners, leucine-rich repeat (LRR-1) protein and proteasome subunit beta 4 (PBS-4), pointing to its involvement in the ubiquitin–proteasome pathway. View Full-Text
Keywords: nucleus; phosphatidylinositol 4,5-bisphosphate; PPK-1; C. elegans nucleus; phosphatidylinositol 4,5-bisphosphate; PPK-1; C. elegans
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Ulicna, L.; Rohozkova, J.; Hozak, P. Multiple Aspects of PIP2 Involvement in C. elegans Gametogenesis. Int. J. Mol. Sci. 2018, 19, 2679.

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