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Transcriptomic Characterization of Bradyrhizobium diazoefficiens Bacteroids Reveals a Post-Symbiotic, Hemibiotrophic-Like Lifestyle of the Bacteria within Senescing Soybean Nodules
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Int. J. Mol. Sci. 2018, 19(12), 3947; https://doi.org/10.3390/ijms19123947

Proteomic Characterization of Bradyrhizobium diazoefficiens Bacteroids Reveals a Post-Symbiotic, Hemibiotrophic-Like Lifestyle of the Bacteria within Senescing Soybean Nodules

1
Department of Science, Columbia College, Columbia, MO 65216, USA
2
Department of Biochemistry, University of Missouri, Columbia, MO 65211, USA
3
Charles River Labs, Carson City, NV 89523, USA
*
Author to whom correspondence should be addressed.
Received: 8 October 2018 / Revised: 26 November 2018 / Accepted: 29 November 2018 / Published: 8 December 2018
(This article belongs to the Special Issue Biological Nitrogen Fixation)
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Abstract

The form and physiology of Bradyrhizobium diazoefficiens after the decline of symbiotic nitrogen fixation has been characterized. Proteomic analyses showed that post-symbiotic B. diazoefficiens underwent metabolic remodeling as well-defined groups of proteins declined, increased or remained unchanged from 56 to 119 days after planting, suggesting a transition to a hemibiotrophic-like lifestyle. Enzymatic analysis showed distinct patterns in both the cytoplasm and the periplasm. Similar to the bacteroid, the post-symbiotic bacteria rely on a non-citric acid cycle supply of succinate and, although viable, they did not demonstrate the ability to grow within the senescent nodule. View Full-Text
Keywords: Bradyrhizobium diazoefficiens; soybean; Glycine max; nitrogen fixation; senescence; proteomics; hemibiotroph Bradyrhizobium diazoefficiens; soybean; Glycine max; nitrogen fixation; senescence; proteomics; hemibiotroph
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Strodtman, K.N.; Frank, S.; Stevenson, S.; Thelen, J.J.; Emerich, D.W. Proteomic Characterization of Bradyrhizobium diazoefficiens Bacteroids Reveals a Post-Symbiotic, Hemibiotrophic-Like Lifestyle of the Bacteria within Senescing Soybean Nodules. Int. J. Mol. Sci. 2018, 19, 3947.

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