Oleuropein Induces AMPK-Dependent Autophagy in NAFLD Mice, Regardless of the Gender
AbstractOleuropein (Ole) is one of the most plentiful phenolic compounds with antioxidant, anti-inflammatory, anti-atherogenic, hypoglycemic and hypolipidemic effects. The aim of our study was to establish whether the positive Ole-related effects on liver steatosis could be associated with autophagy. Female and male C57BL/6J mice were fed normal diet (ND) or high-fat diet (HFD) for eight weeks, and Ole was added or not for the following eight weeks. The autophagy-related proteins Akt, mTOR, AMPK, ULK1, Beclin-1, LC3B and p62/Sqstm1 were analyzed. Interestingly, Ole induced a different regulation of the Akt/mTOR pathway in female compared to male mice, but was able to activate the autophagic process in ND and HFD mice through AMPK-dependent phosphorylation of ULK1 at Ser555, regardless of the gender. Our work reveals the ability of Ole to induce, in liver of ND and HFD mice, autophagy independently by gender-specific mTOR activation. We highlight Ole as a novel therapeutic approach to counteract unhealthy diet-related liver steatosis by targeting autophagy. View Full-Text
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Porcu, C.; Sideri, S.; Martini, M.; Cocomazzi, A.; Galli, A.; Tarantino, G.; Balsano, C. Oleuropein Induces AMPK-Dependent Autophagy in NAFLD Mice, Regardless of the Gender. Int. J. Mol. Sci. 2018, 19, 3948.
Porcu C, Sideri S, Martini M, Cocomazzi A, Galli A, Tarantino G, Balsano C. Oleuropein Induces AMPK-Dependent Autophagy in NAFLD Mice, Regardless of the Gender. International Journal of Molecular Sciences. 2018; 19(12):3948.Chicago/Turabian Style
Porcu, Cristiana; Sideri, Silvia; Martini, Maurizio; Cocomazzi, Alessandra; Galli, Andrea; Tarantino, Giovanni; Balsano, Clara. 2018. "Oleuropein Induces AMPK-Dependent Autophagy in NAFLD Mice, Regardless of the Gender." Int. J. Mol. Sci. 19, no. 12: 3948.
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