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Int. J. Mol. Sci. 2019, 20(2), 235; https://doi.org/10.3390/ijms20020235

Expression Analysis of PIN Genes in Root Tips and Nodules of Lotus japonicus

Department of Botany, Faculty of Agriculture and Biology, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, Poland
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Received: 7 November 2018 / Revised: 31 December 2018 / Accepted: 4 January 2019 / Published: 9 January 2019
(This article belongs to the Special Issue Biological Nitrogen Fixation)
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Abstract

Auxins are postulated to be one of the pivotal factors in nodulation. However, their transporters in Lotus japonicus, the model species for the study of the development of determinate-type root nodules, have been scarcely described so far, and thus their role in nodulation has remained unknown. Our research is the first focusing on polar auxin transporters in L. japonicus. We analyzed and compared expression of PINs in 20 days post rhizobial inoculation (dpi) and 54 dpi root nodules of L. japonicus by real-time quantitative polymerase chain reaction (qPCR) along with the histochemical β-glucuronidase (GUS) reporter gene assay in transgenic hairy roots. The results indicate that LjPINs are essential during root nodule development since they are predominantly expressed in the primordia and young, developing nodules. However, along with differentiation, expression levels of several PINs decreased and occurred particularly in the nodule vascular bundles, especially in connection with the root’s stele. Moreover, our study demonstrated the importance of both polar auxin transport and auxin intracellular homeostasis during L. japonicus root nodule development and differentiation. View Full-Text
Keywords: Lotus japonicus; root nodule; PIN-protein; auxin Lotus japonicus; root nodule; PIN-protein; auxin
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Sańko-Sawczenko, I.; Dmitruk, D.; Łotocka, B.; Różańska, E.; Czarnocka, W. Expression Analysis of PIN Genes in Root Tips and Nodules of Lotus japonicus. Int. J. Mol. Sci. 2019, 20, 235.

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