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PAX2 Expression in Ovarian Cancer
Department of Gynecologic Oncology and Reproductive Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
Cancer Biology Program, The University of Texas at Houston Graduate School of Biomedical Sciences, Houston, TX 77030, USA
* Author to whom correspondence should be addressed.
Received: 25 January 2013; in revised form: 5 March 2013 / Accepted: 13 March 2013 / Published: 15 March 2013
Abstract: PAX2 is one of nine PAX genes that regulate tissue development and cellular differentiation in embryos. However, the functional role of PAX2 in ovarian cancer is not known. Twenty-six ovarian cancer cell lines with different histology origins were screened for PAX2 expression. Two ovarian cancer cell lines: RMUGL (mucinous) and TOV21G (clear cell), with high PAX2 expression were chosen for further study. Knockdown PAX2 expression in these cell lines was achieved by lentiviral shRNAs targeting the PAX2 gene. PAX2 stable knockdown cells were characterized for cell proliferation, migration, apoptosis, protein profiles, and gene expression profiles. The result indicated that these stable PAX2 knockdown cells had reduced cell proliferation and migration. Microarray analysis indicated that several genes involved in growth inhibition and motility, such as G0S2, GREM1, and WFDC1, were up-regulated in PAX2 knockdown cells. On the other hand, over-expressing PAX2 in PAX2-negative ovarian cell lines suppressed their cell proliferation. In summary, PAX2 could have both oncogenic and tumor suppression functions, which might depend on the genetic content of the ovarian cancer cells. Further investigation of PAX2 in tumor suppression and mortality is warranty.
Keywords: PAX2; ovarian cancer; G0S2; WFDC1; GREM1; shRNA
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Song, H.; Kwan, S.-Y.; Izaguirre, D.I.; Zu, Z.; Tsang, Y.T.; Tung, C.S.; King, E.R.; Mok, S.C.; Gershenson, D.M.; Wong, K.-K. PAX2 Expression in Ovarian Cancer. Int. J. Mol. Sci. 2013, 14, 6090-6105.
Song H, Kwan S-Y, Izaguirre DI, Zu Z, Tsang YT, Tung CS, King ER, Mok SC, Gershenson DM, Wong K-K. PAX2 Expression in Ovarian Cancer. International Journal of Molecular Sciences. 2013; 14(3):6090-6105.
Song, Huijuan; Kwan, Suet-Yan; Izaguirre, Daisy I.; Zu, Zhifei; Tsang, Yvonne T.; Tung, Celestine S.; King, Erin R.; Mok, Samuel C.; Gershenson, David M.; Wong, Kwong-Kwok. 2013. "PAX2 Expression in Ovarian Cancer." Int. J. Mol. Sci. 14, no. 3: 6090-6105.