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Article

Signature Ions in MS/MS Spectra for Dansyl-Aminohexyl-QQIV Adducts on Lysine

Eppley Institute, University of Nebraska Medical Center, Omaha, NE 68198, USA
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Author to whom correspondence should be addressed.
Academic Editor: Zrinka Kovarik
Molecules 2020, 25(11), 2659; https://doi.org/10.3390/molecules25112659
Received: 12 May 2020 / Revised: 2 June 2020 / Accepted: 5 June 2020 / Published: 8 June 2020
(This article belongs to the Special Issue Enzymes Reacting with Organophosphorus Compounds)
Bacterial transglutaminase was used to label human plasma proteins with fluorescent tags. Protein lysines were modified with dansyl-epsilon-aminohexyl-Gln-Gln-Ile-Val-OH (dansylQQIV), while protein glutamines were modified with dansyl cadaverine. Labeled proteins included human butyrylcholinesterase, apolipoprotein A-1, haptoglobin, haptoglobin-related protein, immunoglobulin heavy chain, and hemopexin. Tryptic peptides were analyzed by LC-MS/MS on an Orbitrap Fusion Lumos mass spectrometer. Modified residues were identified in Protein Prospector and Proteome Discoverer searches of mass spectrometry data. The MS/MS fragmentation spectra from dansylQQIV-modified peptides gave intense peaks at 475.2015, 364.1691, 347.1426, 234.0585, and 170.0965 m/z. These signature ions are useful markers for identifying modified peptides. Human butyrylcholinesterase retained full activity following modification by dansylQQIV or dansyl cadaverine. View Full-Text
Keywords: mass spectrometry; Protein Prospector; transglutaminase; dansylQQIV; butyrylcholinesterase; plasma proteins mass spectrometry; Protein Prospector; transglutaminase; dansylQQIV; butyrylcholinesterase; plasma proteins
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MDPI and ACS Style

Schopfer, L.M.; Lockridge, O. Signature Ions in MS/MS Spectra for Dansyl-Aminohexyl-QQIV Adducts on Lysine. Molecules 2020, 25, 2659. https://doi.org/10.3390/molecules25112659

AMA Style

Schopfer LM, Lockridge O. Signature Ions in MS/MS Spectra for Dansyl-Aminohexyl-QQIV Adducts on Lysine. Molecules. 2020; 25(11):2659. https://doi.org/10.3390/molecules25112659

Chicago/Turabian Style

Schopfer, Lawrence M.; Lockridge, Oksana. 2020. "Signature Ions in MS/MS Spectra for Dansyl-Aminohexyl-QQIV Adducts on Lysine" Molecules 25, no. 11: 2659. https://doi.org/10.3390/molecules25112659

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