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Phytoestrogenic Effects of Blackcurrant Anthocyanins Increased Endothelial Nitric Oxide Synthase (eNOS) Expression in Human Endothelial Cells and Ovariectomized Rats
Open AccessArticle

Blackcurrant Extract with Phytoestrogen Activity Alleviates Hair Loss in Ovariectomized Rats

by Naoki Nanashima *,† and Kayo Horie
Department of Bioscience and Laboratory Medicine, Hirosaki University Graduate School of Health Sciences, 66-1 Hon-cho, Hirosaki, Aomori 036-8564, Japan
*
Author to whom correspondence should be addressed.
These authors contributed equally to this manuscript.
Academic Editors: Takanori Tsuda and Wilhelmina Kalt
Molecules 2019, 24(7), 1272; https://doi.org/10.3390/molecules24071272
Received: 19 February 2019 / Revised: 19 March 2019 / Accepted: 21 March 2019 / Published: 1 April 2019
(This article belongs to the Special Issue Anthocyanins: Recent Progress in Health Benefits Studies)
Ancocyanin-rich blackcurrant extract (BCE) has phytoestrogen activity; however, its effect on hair follicles is unknown. Additionally, hair loss is known to occur during menopause in women owing to decreased estrogen secretion. This study examined whether BCE alleviated female pattern hair loss using a rat model. RNA was extracted and analyzed using a microarray and ingenuity pathway analysis. A quantitative polymerase chain reaction revealed that 1 μg/mL BCE altered many genes downstream of beta-estradiol in human hair dermal papilla cells. Additionally, the expression of the hair follicle stem cell marker keratin 19 was greatly enhanced. In a menopause model, ovariectomized rats were fed a diet containing 3% BCE for three months. An analysis of the number of hair shafts revealed that BCE increased the number of hairs by 0.5 hairs/follicular unit. Moreover, immunostaining revealed that the expression of Ki67 also increased by 19%. Furthermore, fluorescent immunostaining showed that the expression of other stem cell markers, including keratin 15, CD34, and keratin 19, was induced in rat hair follicular cells. In conclusion, these findings suggest that BCE has phytoestrogen activity in hair follicles and contributes to the alleviation of hair loss in a menopausal model in rats. View Full-Text
Keywords: ancocyanin; blackcurrant; female pattern hair loss; ovariectomized rat; phytoestrogen ancocyanin; blackcurrant; female pattern hair loss; ovariectomized rat; phytoestrogen
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Nanashima, N.; Horie, K. Blackcurrant Extract with Phytoestrogen Activity Alleviates Hair Loss in Ovariectomized Rats. Molecules 2019, 24, 1272.

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