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Article

In Vitro Enzymatic Digestibility of Glutaraldehyde-Crosslinked Chitosan Nanoparticles in Lysozyme Solution and Their Applicability in Pulmonary Drug Delivery

1
Pharmacy Discipline, School of Clinical Sciences, Faculty of Health, Queensland University of Technology (QUT), Brisbane, QLD 4000, Australia
2
Institute of Health and Biomedical Innovation, QUT, 60 Musk Avenue, Kelvin Grove, Brisbane, QLD 4059, Australia
3
School of Pharmacy, The University of Queensland, Woolloongabba, QLD 4102, Brisbane, Australia
4
School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia
5
Australian Infectious Diseases Research Centre, The University of Queensland, Brisbane, QLD 4072, Australia
*
Author to whom correspondence should be addressed.
Molecules 2019, 24(7), 1271; https://doi.org/10.3390/molecules24071271
Received: 20 March 2019 / Revised: 27 March 2019 / Accepted: 28 March 2019 / Published: 1 April 2019
(This article belongs to the Special Issue Chitosan-Based Nanomaterials for Biomedical Applications)
Herein, the degradation of low molecular weight chitosan (CS), with 92% degree of deacetylation (DD), and its nanoparticles (NP) has been investigated in 0.2 mg/mL lysozyme solution at 37 °C. The CS nanoparticles were prepared using glutaraldehyde crosslinking of chitosan in a water-in-oil emulsion system. The morphological characterization of CS particles was carried out using scanning electron microscopy (SEM) and Transmission Electron Microscopy (TEM) techniques. Using attenuated total reflectance Fourier transform infrared (ATR-FTIR) and UV-VIS spectroscopy, the structural integrity of CS and its NPs in lysozyme solution were monitored. The CS powder showed characteristic FTIR bands around 1150 cm−1 associated with the glycosidic bridges (C-O-C bonds) before and after lysozyme treatment for 10 weeks, which indicated no CS degradation. The glutaraldehyde crosslinked CS NPs showed very weak bands associated with the glycosidic bonds in lysozyme solution. Interestingly, the UV-VIS spectroscopic data showed some degradation of CS NPs in lysozyme solution. The results of this study indicate that CS with a high DD and its NPs crosslinked with glutaraldehyde were not degradable in lysozyme solution and thus unsuitable for pulmonary drug delivery. Further studies are warranted to understand the complete degradation of CS and its NPs to ensure their application in pulmonary drug delivery. View Full-Text
Keywords: chitosan; glutaraldehyde; nanoparticles; lysozyme; degradation; pulmonary drug delivery chitosan; glutaraldehyde; nanoparticles; lysozyme; degradation; pulmonary drug delivery
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MDPI and ACS Style

Islam, N.; Wang, H.; Maqbool, F.; Ferro, V. In Vitro Enzymatic Digestibility of Glutaraldehyde-Crosslinked Chitosan Nanoparticles in Lysozyme Solution and Their Applicability in Pulmonary Drug Delivery. Molecules 2019, 24, 1271. https://doi.org/10.3390/molecules24071271

AMA Style

Islam N, Wang H, Maqbool F, Ferro V. In Vitro Enzymatic Digestibility of Glutaraldehyde-Crosslinked Chitosan Nanoparticles in Lysozyme Solution and Their Applicability in Pulmonary Drug Delivery. Molecules. 2019; 24(7):1271. https://doi.org/10.3390/molecules24071271

Chicago/Turabian Style

Islam, Nazrul, Hui Wang, Faheem Maqbool, and Vito Ferro. 2019. "In Vitro Enzymatic Digestibility of Glutaraldehyde-Crosslinked Chitosan Nanoparticles in Lysozyme Solution and Their Applicability in Pulmonary Drug Delivery" Molecules 24, no. 7: 1271. https://doi.org/10.3390/molecules24071271

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