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Molecules 2019, 24(4), 680;

Identification and Extraction Optimization of Active Constituents in Citrus junos Seib ex TANAKA Peel and Its Biological Evaluation

Department of Pharmacy, College of Pharmacy, Mokpo National University, Muan, Jeonnam 58554, Korea
Department of Dental Pharmacology, School of Dentistry and Institute of Oral Bioscience, BK21 Plus, Chonbuk National University, Jeonju 56443, Korea
Authors to whom correspondence should be addressed.
Academic Editors: In-Soo Yoon, Hyun-Jong Cho and Derek J. McPhee
Received: 27 January 2019 / Revised: 12 February 2019 / Accepted: 13 February 2019 / Published: 14 February 2019
(This article belongs to the Special Issue Method Development and Validation in Food and Pharmaceutical Analysis)
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Citrus junos Seib ex TANAKA possesses various biological effects. It has been used in oriental remedies for blood circulation and the common cold. Recently, biological effects of C. junos peel have been reported. However, optimization of the biological properties of C. junos peel preparations has yet to be reported on. We developed a high-performance liquid chromatography (HPLC) method for quantification of the active constituents in C. junos peel. Hot water and ethanolic extracts of C. junos peel were prepared and their chemical profiles and biological activities were evaluated. The 80% ethanolic extract demonstrated the greatest antioxidant activity and phenolic content, while the 100% ethanolic extract had the greatest xanthine oxidase inhibitory activity. Elastase inhibition activity was superior in aqueous and 20% ethanolic extracts. The contents of two flavonoids were highest in the 100% ethanolic extract. We postulated that the antioxidant and anti-aging effects of C. junos peel extract could be attributed to phenolics such as flavonoids. Our results suggest that the flavonoid-rich extract of C. junos may be utilized for the treatment and prevention of metabolic disease and hyperuricemia while the water-soluble extract of C. junos could be used as a source for its anti-aging properties. View Full-Text
Keywords: Citrus junos Seib ex TANAKA; antioxidant; xanthine oxidase; elastase Citrus junos Seib ex TANAKA; antioxidant; xanthine oxidase; elastase

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Shim, J.-H.; Chae, J.-I.; Cho, S.-S. Identification and Extraction Optimization of Active Constituents in Citrus junos Seib ex TANAKA Peel and Its Biological Evaluation. Molecules 2019, 24, 680.

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