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Open AccessArticle

Rapid Characterization of Triterpene Saponins from Zornia brasiliensis by HPLC-ESI-MS/MS

Graduate Program in Natural and Synthetic Bioactive Products, Health Sciences Center, Universidade Federal da Paraíba, João Pessoa 58051-900, Paraíba, Brazil
Graduate Program in Ecology and Conservation, Department of Biology, Center for Biological and Health Sciences, Campina Grande 58429-500, Paraíba, Brazil
Center of Sciences and Technologies, Darcy Ribeiro Norte Fluminense State University, Campos dos Goytacazes 28013-600, Rio de Janeiro, Brazil
Author to whom correspondence should be addressed.
Academic Editors: David Popovich and Valeria Patricia Sülsen
Molecules 2019, 24(14), 2519;
Received: 2 May 2019 / Revised: 6 July 2019 / Accepted: 9 July 2019 / Published: 10 July 2019
(This article belongs to the Special Issue Saponins)
PDF [1333 KB, uploaded 10 July 2019]


Zornia brasiliensis Vogel (Leguminosae) is a species popularly known in Brazil as “urinária”, “urinana”, and “carrapicho”, it is popularly used as a diuretic and in the treatment of venereal diseases. A specific methodology to obtain a saponin-enriched fraction and high-performance liquid chromatography coupled with diode array detection, ion trap mass spectrometry, and TOF-MS (HPLC-DAD-ESI-MS/MS) was applied for the analysis of triterpene saponins. The MS and MS/MS experiments were carried out by ionization in negative mode. Molecular mass and fragmentation data were used to support the structural characterization of the saponins. Based on retention times, high-resolution mass determination and fragmentation, 35 oleanane-triterpene saponins were tentatively identified in Z. brasiliensis. View Full-Text
Keywords: triterpene saponins; soyasaponins; Zornia brasiliensis; HPLC-DAD-ESI-MS/MS triterpene saponins; soyasaponins; Zornia brasiliensis; HPLC-DAD-ESI-MS/MS

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Nascimento, Y.M.; Abreu, L.S.; Lima, R.L.; Costa, V.C.O.; Melo, J.I.M.; Braz-Filho, R.; Silva, M.S.; Tavares, J.F. Rapid Characterization of Triterpene Saponins from Zornia brasiliensis by HPLC-ESI-MS/MS. Molecules 2019, 24, 2519.

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