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Open AccessArticle

Inhibitory Activity of Plant Essential Oils against E. coli 1-Deoxy-d-xylulose-5-phosphate reductoisomerase

1
National Engineering Research Center for Miniaturized Detection Systems and College of Life Sciences, Northwest University, 229 North Taibai Road, Xi’an 710069, China
2
School of Pharmacy, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, China
*
Authors to whom correspondence should be addressed.
G.Y. and B.-R.Z. contributed equally.
Academic Editor: Isabel C.F.R. Ferreira
Molecules 2019, 24(14), 2518; https://doi.org/10.3390/molecules24142518
Received: 10 June 2019 / Revised: 1 July 2019 / Accepted: 3 July 2019 / Published: 10 July 2019
(This article belongs to the Collection Bioactive Compounds)
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Abstract

The rate-limiting enzyme of the 2-methyl-d-erythritol-4-phosphate (MEP) terpenoid biosynthetic pathway, 1-deoxy-d-xylulose-5-phosphate reductoisomerase (DXR), provides the perfect target for screening new antibacterial substances. In this study, we tested the DXR inhibitory effect of 35 plant essential oils (EOs), which have long been recognized for their antimicrobial properties. The results show that the EOs of Zanbthoxylum bungeanum (ZB), Schizonepetae tenuifoliae (ST), Thymus quinquecostatus (TQ), Origanum vulgare (OV), and Eugenia caryophyllata (EC) displayed weak to medium inhibitory activity against DXR, with IC50 values of 78 μg/mL, 65 μg/mL, 59 μg/mL, 48 μg/mL, and 37 μg/mL, respectively. GC-MS analyses of the above oils and further DXR inhibitory activity tests of their major components revealed that eugenol (EC) and carvacrol (TQ and OV) possess medium inhibition against the protein (68.3% and 55.6%, respectively, at a concentration of 20 μg/mL), whereas thymol (ST, TQ, and OV), carveol (ZB), and linalool (ZB, ST, and OV) only exhibited weak inhibition against DXR, at 20 μg/mL (23%−26%). The results add more details to the antimicrobial mechanisms of plant EOs, which could be very helpful in the direction of the reasonable use of EOs in the food industry and in the control of phytopathogenic microbials. View Full-Text
Keywords: plant essential oils; antibacterial mechanism; 1-deoxy-d-xylulose-5-phosphate reductoisomerase; inhibition plant essential oils; antibacterial mechanism; 1-deoxy-d-xylulose-5-phosphate reductoisomerase; inhibition
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Yan, G.; Zhu, B.-R.; Tian, F.-L.; Hui, X.; Li, H.; Li, Y.-M.; Gao, W.-Y. Inhibitory Activity of Plant Essential Oils against E. coli 1-Deoxy-d-xylulose-5-phosphate reductoisomerase. Molecules 2019, 24, 2518.

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