Next Article in Journal
Effects of Portulaca Oleracea Extract on Acute Alcoholic Liver Injury of Rats
Next Article in Special Issue
Neuroprotective Effects of Ginseng Phytochemicals: Recent Perspectives
Previous Article in Journal
Green Synthesis of Privileged Benzimidazole Scaffolds Using Active Deep Eutectic Solvent
Previous Article in Special Issue
Rapid Characterization of Triterpene Saponins from Zornia brasiliensis by HPLC-ESI-MS/MS
Article Menu

Export Article

Open AccessArticle

Ophiopogon Polysaccharide Promotes the In Vitro Metabolism of Ophiopogonins by Human Gut Microbiota

Shenzhen Key Laboratory of Edible and Medicinal Bioresources, HKUST Shenzhen Research Institute, Shenzhen 518057, China
Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Hong Kong 999077, China
Author to whom correspondence should be addressed.
Academic Editor: David Popovich
Molecules 2019, 24(16), 2886;
Received: 12 July 2019 / Revised: 6 August 2019 / Accepted: 8 August 2019 / Published: 8 August 2019
(This article belongs to the Special Issue Saponins)
PDF [1207 KB, uploaded 8 August 2019]
  |     |  


Gut microbiota play an important role in metabolism of intake saponins, and parallelly, the polysaccharides deriving from herbal products possess effects on gut microbiota. Ophiopogonis Radix is a common Chinese herb that is popularly used as functional food in China. Polysaccharide and steroidal saponin, e.g., ophiopogonin, mainly ophiopogonin D (Oph-D) and ophiopogonin D’ (Oph-D’), are the major constituents in this herb. In order to reveal the role of gut microbiota in metabolizing ophiopogonin, an in vitro metabolism of Oph-D and Oph-D’ by human gut microbiota, in combination with or without Ophiopogon polysaccharide, was conducted. A sensitive and reliable UPLC-MS/MS method was developed to simultaneously quantify Oph-D, Oph-D’ and their final metabolites, i.e., ruscogenin and diosgenin in the broth of microbiota. An elimination of Oph-D and Oph-D’ was revealed in a time-dependent manner, as well as the recognition of a parallel increase of ruscogenin and diosgenin. Ophiopogon polysaccharide was shown to stimulate the gut microbiota-induced metabolism of ophiopogonins. This promoting effect was further verified by increased activities of β-D-glucosidase, β-D-xylosidase, α-L-rhamnosidase and β-D-fucosidase in the broth. This study can be extended to investigate the metabolism of steroidal saponins by gut microbiota when combined with other herbal products, especially those herbs enriched with polysaccharides. View Full-Text
Keywords: ophiopogonin; human gut microbiota; LC-MS/MS; Ophiopogon polysaccharide; promotion effects ophiopogonin; human gut microbiota; LC-MS/MS; Ophiopogon polysaccharide; promotion effects

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Wang, H.-Y.; Guo, S.-C.; Peng, Z.-T.; Wang, C.; Duan, R.; Dong, T.T.X.; Tsim, K.W.K. Ophiopogon Polysaccharide Promotes the In Vitro Metabolism of Ophiopogonins by Human Gut Microbiota. Molecules 2019, 24, 2886.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top