Next Article in Journal
Remembering Professor Benito Casu (1927–2016)
Next Article in Special Issue
Practical Application of Aptamer-Based Biosensors in Detection of Low Molecular Weight Pollutants in Water Sources
Previous Article in Journal
Enzymatic Synthesis of Amino Acids Endcapped Polycaprolactone: A Green Route Towards Functional Polyesters
Previous Article in Special Issue
Development of Cell-Specific Aptamers: Recent Advances and Insight into the Selection Procedures
Article Menu
Issue 2 (February) cover image

Export Article

Open AccessArticle

A Lateral Flow Strip Based Aptasensor for Detection of Ochratoxin A in Corn Samples

Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China
College of Food Science and Technology, Hainan University, Haikou 570228, China
Author to whom correspondence should be addressed.
Molecules 2018, 23(2), 291;
Received: 19 December 2017 / Revised: 11 January 2018 / Accepted: 15 January 2018 / Published: 31 January 2018
(This article belongs to the Special Issue Nucleic Acid Aptamers)
PDF [1640 KB, uploaded 31 January 2018]


Ochratoxin A (OTA) is a mycotoxin identified as a contaminant in grains and wine throughout the world, and convenient, rapid and sensitive detection methods for OTA have been a long-felt need for food safety monitoring. Herein, we presented a new competitive format based lateral flow strip fluorescent aptasensor for one-step determination of OTA in corn samples. Briefly, biotin-cDNA was immobilized on the surface of a nitrocellulose filter on the test line. Without OTA, Cy5-labeled aptamer combined with complementary strands formed a stable double helix. In the presence of OTA, however, the Cy5-aptamer/OTA complexes were generated, and therefore less free aptamer was captured in the test zone, leading to an obvious decrease in fluorescent signals on the test line. The test strip showed an excellent linear relationship in the range from 1 ng·mL−1 to 1000 ng·mL−1 with the LOD of 0.40 ng·mL−1, IC15 value of 3.46 ng·mL−1 and recoveries from 96.4% to 104.67% in spiked corn samples. Thus, the strip sensor developed in this study is an acceptable alternative for rapid detection of the OTA level in grain samples. View Full-Text
Keywords: aptamer; lateral flow strip; detection; Ochratoxin A; corn samples aptamer; lateral flow strip; detection; Ochratoxin A; corn samples

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Zhang, G.; Zhu, C.; Huang, Y.; Yan, J.; Chen, A. A Lateral Flow Strip Based Aptasensor for Detection of Ochratoxin A in Corn Samples. Molecules 2018, 23, 291.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top