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Development of Cell-Specific Aptamers: Recent Advances and Insight into the Selection Procedures

Centre for comparative Genomics, Murdoch University, Perth 6150, Australia
Perron Institute for Neurological and Translational Science, Perth 6005, Australia
School of Medicine, Deakin University, Waurn Ponds, Geelong 3216, Australia
Author to whom correspondence should be addressed.
Molecules 2017, 22(12), 2070;
Received: 7 November 2017 / Revised: 24 November 2017 / Accepted: 24 November 2017 / Published: 27 November 2017
(This article belongs to the Special Issue Nucleic Acid Aptamers)
Systematic evolution of ligands by exponential enrichment (SELEX) is an established procedure for developing short single-stranded nucleic acid ligands called aptamers against a target of choice. This approach has also been used for developing aptamers specific to whole cells named Cell-SELEX. Aptamers selected by Cell-SELEX have the potential to act as cell specific therapeutics, cell specific markers or cell specific drug delivery and imaging agents. However, aptamer development is a laborious and time-consuming process which is often challenging due to the requirement of frequent optimization of various steps involved in Cell-SELEX procedures. This review provides an insight into various procedures for selection, aptamer enrichment, regeneration and aptamer-binding analysis, in addition to a very recent update on all aptamers selected by Cell-SELEX procedures. View Full-Text
Keywords: aptamers; Cell-SELEX; aptamer enrichment; ssDNA; binding assays aptamers; Cell-SELEX; aptamer enrichment; ssDNA; binding assays
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Rahimizadeh, K.; AlShamaileh, H.; Fratini, M.; Chakravarthy, M.; Stephen, M.; Shigdar, S.; Veedu, R.N. Development of Cell-Specific Aptamers: Recent Advances and Insight into the Selection Procedures. Molecules 2017, 22, 2070.

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