Next Article in Journal
Biotinylated Cyclooligosaccharides for Paclitaxel Solubilization
Previous Article in Journal
The Impact of Lipid Types and Liposomal Formulations on Osteoblast Adiposity and Mineralization
Article Menu
Issue 1 (January) cover image

Export Article

Open AccessArticle
Molecules 2018, 23(1), 91; https://doi.org/10.3390/molecules23010091

Immunosuppressive Effect of Geniposide on Mitogen-Activated Protein Kinase Signalling Pathway and Their Cross-Talk in Fibroblast-Like Synoviocytes of Adjuvant Arthritis Rats

1
College of Pharmacy, Anhui University of Chinese Medicine, Key Laboratory of Modernized Chinese, Medicine in Anhui Province, Hefei 230012, Anhui, China
2
Hefei Anderson Pharmaceutical Co., Ltd., Hefei 230088, Anhui, China
Feng Li and Miao-Miao Dai contributed equally to this work
*
Author to whom correspondence should be addressed.
Received: 24 November 2017 / Revised: 27 December 2017 / Accepted: 28 December 2017 / Published: 2 January 2018
Full-Text   |   PDF [4258 KB, uploaded 23 January 2018]   |  

Abstract

Geniposide (GE), an iridoid glycoside compound derived from Gardenia jasminoides Ellis fruit, is known to have anti-inflammatory and immunoregulatory activities. The aim of this study was to investigate the protective mechanism of GE in the regulation of the mitogen-activated protein kinase (MAPK) signalling pathway and the cross-talk among the MAPK signalling pathway in fibroblast-like synoviocytes (FLS) of adjuvant arthritis (AA) rats. AA was induced by injecting with Freund’s complete adjuvant. Male SD rats and FLS were subjected to treatment with GE (30, 60 and 120 mg/kg) in vivo from day 14 to 21 after immunization and GE (25, 50 and 100 μg/mL) in vitro, respectively. The proliferation of FLS was assessed by MTT. IL-4, IL-17, IFN-γ, and TGF-β1 were determined by ELISA. Key proteins in the MAPK signalling pathway were detected by Western blot. GE significantly reduced the proliferation of FLS, along with decreased IFN-γ and IL-17 and increased IL-4 and TGF-β1. In addition, GE decreased the expression of p-JNK, p-ERK1/2 and p-p38 in FLS of AA rats. Furthermore, disrupting one MAPK pathway inhibited the activation of other MAPK pathways, suggesting cross-talk among MAPK signalling. In vivo study, it was also observed that GE attenuated histopathologic changes in the synovial tissue of AA rats. Collectively, the mechanisms by which GE exerts anti-inflammatory and immunoregulatory effects may be related to the synergistic effect of JNK, ERK1/2 and p38. Targeting MAPK signalling may be a new therapeutic strategy in inflammatory/autoimmune diseases. View Full-Text
Keywords: geniposide; adjuvant arthritis; MAPK signalling; cross-talk; fibroblast-like synoviocytes; rheumatoid arthritis geniposide; adjuvant arthritis; MAPK signalling; cross-talk; fibroblast-like synoviocytes; rheumatoid arthritis
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Li, F.; Dai, M.; Wu, H.; Deng, R.; Fu, J.; Zhang, Z.; Dai, L.; Wang, W.; Dai, X.; Zhan, X.; Wang, Y. Immunosuppressive Effect of Geniposide on Mitogen-Activated Protein Kinase Signalling Pathway and Their Cross-Talk in Fibroblast-Like Synoviocytes of Adjuvant Arthritis Rats. Molecules 2018, 23, 91.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top